Literature summary for 5.4.99.60 extracted from

Xue, Y.; Wei, Z.; Li, X.; Gong, W.
The crystal structure of putative precorrin isomerase CbiC in cobalamin biosynthesis (2006), J. Struct. Biol., 153, 307-311.

Search for more literature for 5.4.99.60

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Leptospira interrogans
expression in Escherichia coli as His-tagged protein	Leptospira interrogans

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using either 10% (v/v) PEG6000, 2.0 M sodium chloride or 20% (v/v) ethanol, 0.1 M Tris, pH 8.5	Leptospira interrogans
two crystal forms of space groups P42212 and P3121, diffracting to 3.0 and 2.3 A resolution, resp.	Leptospira interrogans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cobalt-precorrin-8	Leptospira interrogans	-	cobyrinate	-	?

Organism

Organism	UniProt	Comment	Textmining
Leptospira interrogans	-	-	-
Leptospira interrogans	Q8EXP7	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-affinity column chromatography	Leptospira interrogans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cobalt-precorrin-8	-	Leptospira interrogans	cobyrinate	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Leptospira interrogans

Synonyms

Synonyms	Comment	Organism
CbiC	-	Leptospira interrogans
cobalt-precorrin 8 isomerase	-	Leptospira interrogans
Precorrin isomerase	-	Leptospira interrogans

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)