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Literature summary for 5.4.99.59 extracted from
- Characterization of the dTDP-D-fucofuranose biosynthetic pathway in Escherichia coli O52 (2008), Mol. Microbiol., 70, 1358-1367.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli BL21 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 5 mM, complete inhibition | Escherichia coli |  |
| Mn2+ | 5 mM, 43% inhibition | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.43 | - |
dTDP-alpha-D-fucopyranose | pH 7.4, 37°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Fcf2 is an divalent cation-independent enzyme | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-alpha-D-fucopyranose | Escherichia coli | the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O antigen | dTDP-beta-D-fuco-(1->4)-furanose | - |
? | |
| dTDP-alpha-D-fucopyranose | Escherichia coli O52 | the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O antigen | dTDP-beta-D-fuco-(1->4)-furanose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | Q6E7F1 | - |
- |
| Escherichia coli O52 | Q6E7F1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His6-tagged fusion protein | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 50% glycerol, stable for at least 2 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTDP-alpha-D-fucopyranose | - |
Escherichia coli | dTDP-beta-D-fuco-(1->4)-furanose | - |
? | |
| dTDP-alpha-D-fucopyranose | the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O antigen | Escherichia coli | dTDP-beta-D-fuco-(1->4)-furanose | - |
? | |
| dTDP-alpha-D-fucopyranose | - |
Escherichia coli O52 | dTDP-beta-D-fuco-(1->4)-furanose | - |
? | |
| dTDP-alpha-D-fucopyranose | the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O antigen | Escherichia coli O52 | dTDP-beta-D-fuco-(1->4)-furanose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Fcf2 | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 37 | - |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 50 | 4°C: 17% of maximal activity, 50°C: 49% of maximal activity | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.056 | - |
dTDP-alpha-D-fucopyranose | pH 7.4, 37°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | mutant strains H1863 lacking fcf2. The mutant strain produces semirough lipopolysaccharide with only one O unit attached to the core-lipid A moiety while the wild type strain produces normal lipopolysaccharide. The result indicates that fcf2 is required for the synthesis of the O antigen in Escherichia coli O52 | Escherichia coli |
| physiological function | the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O antigen | Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
dTDP-alpha-D-fucopyranose | pH 7.4, 37°C | Escherichia coli | |
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