Literature summary for 5.4.99.5 extracted from

Krengel, U.; Dey, R.; Sasso, S.; Okvist, M.; Ramakrishnan, C.; Kast, P.
Preliminary X-ray crystallographic analysis of the secreted chorismate mutase from Mycobacterium tuberculosis: a tricky crystallization problem solved (2006), Acta Crystallogr. Sect. F, 62, 441-445.

Cloned(Commentary)

Cloned (Comment)	Organism
*MtCM prepared from Escherichia coli strain KA29	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
MtCM, encoded by ORF Rv1885c in strain H37Rv. First characterized example of an AroQgamma fold. Description of the crystal optimization of a protein target that crystallizes very rapidly. 1. 175-residue version of MtCM (encoded on plasmid pKTU3-HCT, dissolved in 20 mM potassium phosphate buffer pH7.5. 2. leaderless untagged 167-residue version of *MtCM encoded by plasmid pKTU3-HT used, buffered with 20 mM Tris-HCl pH 8.0)	Mycobacterium tuberculosis

Crystallization (Comment)

Organism

*MtCM, encoded by ORF Rv1885c in strain H37Rv. First characterized example of an AroQgamma fold. Description of the crystal optimization of a protein target that crystallizes very rapidly. 1. 175-residue version of *MtCM (encoded on plasmid pKTU3-HCT, dissolved in 20 mM potassium phosphate buffer pH7.5. 2. leaderless untagged 167-residue version of *MtCM encoded by plasmid pKTU3-HT used, buffered with 20 mM Tris-HCl pH 8.0)

Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37200	-	-	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Chorismate	Mycobacterium tuberculosis	-	Prephenate	-	?
Chorismate	Mycobacterium tuberculosis H37Rv	-	Prephenate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WIB9	encoded by Rv1885c	-
Mycobacterium tuberculosis H37Rv	P9WIB9	encoded by Rv1885c	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Chorismate	-	Mycobacterium tuberculosis	Prephenate	-	?
Chorismate	-	Mycobacterium tuberculosis H37Rv	Prephenate	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
chorismate mutase	-	Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	assay at	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Mycobacterium tuberculosis