BRENDA - Enzyme Database
show all sequences of 5.4.99.47

Oxidosqualene Cyclase Residues that Promote Formation of Cycloartenol, Lanosterol, and Parkeol We are grateful to Bridget M. Joubert for advice regarding mutagenesis. We thank Elizabeth A. Hart for an authentic parkeol standard, and for chromatographic and spectroscopic information. This research was funded by the National Institutes of Health (grant no. AI 41598) and the Robert A. Welch Foundation (grant no. C-1323). M.M.M. was an American Society of Pharmacognosy Undergraduate Fellow. M.J.R.S. was a Robert A. Welch Fellow and was supported by an NIH Biotechnology Training Grant (grant no. T32 GM08362).

Khenkin, A.; Neumann, R.; Angew. Chem. 39, 4090-4092 (2000)

Data extracted from this reference:

Organism
Organism
UniProt
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(S)-2,3-oxidosqualene
-
713760
Saccharomyces cerevisiae
parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol
lanosterol-synthase mutant T384Y produces 11% parkeol, 79% lanosterol and 10% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene
-
-
?
(S)-2,3-oxidosqualene
-
713760
Saccharomyces cerevisiae
parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol
lanosterol-synthase mutant T384Y/V4545L produces 60% parkeol, 20% lanosterol and 20% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene
-
-
?
(S)-2,3-oxidosqualene
-
713760
Saccharomyces cerevisiae
parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol
lanosterol-synthase mutant T384Y/V454I produces 64% parkeol, 13% lanosterol and 23% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene
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-
?
Synonyms
Synonyms
Commentary
Organism
lanosterol-synthase mutant T384Y
cf. EC 5.4.99.7
Saccharomyces cerevisiae
lanosterol-synthase mutant T384Y/V454I
cf. EC 5.4.99.7
Saccharomyces cerevisiae
lanosterol-synthase mutant T384Y/V454L
cf. EC 5.4.99.7
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(S)-2,3-oxidosqualene
-
713760
Saccharomyces cerevisiae
parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol
lanosterol-synthase mutant T384Y produces 11% parkeol, 79% lanosterol and 10% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene
-
-
?
(S)-2,3-oxidosqualene
-
713760
Saccharomyces cerevisiae
parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol
lanosterol-synthase mutant T384Y/V4545L produces 60% parkeol, 20% lanosterol and 20% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene
-
-
?
(S)-2,3-oxidosqualene
-
713760
Saccharomyces cerevisiae
parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol
lanosterol-synthase mutant T384Y/V454I produces 64% parkeol, 13% lanosterol and 23% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene
-
-
?
Other publictions for EC 5.4.99.47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748774
Xue
Identification of key amino a ...
Oryza sativa Japonica Group, Oryza sativa Japonica Group Zhonghua 11
New Phytol.
218
1076-1088
2018
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Protein engineering of Sacchar ...
Saccharomyces cerevisiae
Org. Lett.
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5222-5225
2012
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716421
Ito
Triterpene cyclases from Oryza ...
Oryza sativa
Org. Lett.
13
2678-2681
2011
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714021
Kimura
Protostadienol synthase from A ...
Aspergillus fumigatus
Biochem. Biophys. Res. Commun.
391
899-902
2010
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682344
Sawai
Plant lanosterol synthase: div ...
Lotus japonicus
Plant Cell Physiol.
47
673-677
2006
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714739
Wu
Histidine residue at position ...
Saccharomyces cerevisiae
ChemBioChem
6
1177-1181
2005
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653405
Segura
Directed evolution experiments ...
Arabidopsis thaliana
Org. Lett.
4
4459-4462
2002
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713760
Khenkin
Oxidosqualene Cyclase Residues ...
Saccharomyces cerevisiae
Angew. Chem.
39
4090-4092
2000
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715239
Hart
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Directed evolution to investig ...
Saccharomyces cerevisiae
J. Am. Chem. Soc.
121
9887-9888
1999
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