BRENDA - Enzyme Database
show all sequences of 5.4.99.47

Directed evolution experiments reveal mutations at cycloartenol synthase residue His477 that dramatically alter catalysis

Segura, M.J.; Lodeiro, S.; Meyer, M.M.; Patel, A.J.; Matsuda, S.P.; Org. Lett. 4, 4459-4462 (2002)

Data extracted from this reference:

Engineering
Protein Variants
Commentary
Organism
H477N
the mutant produces 88% lanosterol and 12% parkeol from oxidosqualene
Arabidopsis thaliana
H477Q
the mutant produces 73% parkeol, 22% lanosterol, and 5% DELTA7-lanosterol from oxidosqualene
Arabidopsis thaliana
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
oxidosqualene
-
653405
Arabidopsis thaliana
lanosterol + parkeol
mutant H477N produces 88% lanosterol and 12% parkeol
-
-
?
oxidosqualene
-
653405
Arabidopsis thaliana
lanosterol + parkeol
mutant H477Q produces 73% parkeol, 22% lanosterol, and 5% DELTA7-lanosterol
-
-
?
Synonyms
Synonyms
Commentary
Organism
cycloartenol synthase mutant H477N
cf. EC 5.4.99.8
Arabidopsis thaliana
cycloartenol synthase mutant H477Q
cf. EC 5.4.99.8
Arabidopsis thaliana
Engineering (protein specific)
Protein Variants
Commentary
Organism
H477N
the mutant produces 88% lanosterol and 12% parkeol from oxidosqualene
Arabidopsis thaliana
H477Q
the mutant produces 73% parkeol, 22% lanosterol, and 5% DELTA7-lanosterol from oxidosqualene
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
oxidosqualene
-
653405
Arabidopsis thaliana
lanosterol + parkeol
mutant H477N produces 88% lanosterol and 12% parkeol
-
-
?
oxidosqualene
-
653405
Arabidopsis thaliana
lanosterol + parkeol
mutant H477Q produces 73% parkeol, 22% lanosterol, and 5% DELTA7-lanosterol
-
-
?
Other publictions for EC 5.4.99.47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748774
Xue
Identification of key amino a ...
Oryza sativa Japonica Group, Oryza sativa Japonica Group Zhonghua 11
New Phytol.
218
1076-1088
2018
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728425
Liu
Protein engineering of Sacchar ...
Saccharomyces cerevisiae
Org. Lett.
14
5222-5225
2012
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716421
Ito
Triterpene cyclases from Oryza ...
Oryza sativa
Org. Lett.
13
2678-2681
2011
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714021
Kimura
Protostadienol synthase from A ...
Aspergillus fumigatus
Biochem. Biophys. Res. Commun.
391
899-902
2010
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682344
Sawai
Plant lanosterol synthase: div ...
Lotus japonicus
Plant Cell Physiol.
47
673-677
2006
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714739
Wu
Histidine residue at position ...
Saccharomyces cerevisiae
ChemBioChem
6
1177-1181
2005
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653405
Segura
Directed evolution experiments ...
Arabidopsis thaliana
Org. Lett.
4
4459-4462
2002
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2
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713760
Khenkin
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Oxidosqualene cyclase residues ...
Saccharomyces cerevisiae
Angew. Chem.
39
4090-4092
2000
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715239
Hart
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Directed evolution to investig ...
Saccharomyces cerevisiae
J. Am. Chem. Soc.
121
9887-9888
1999
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