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Literature summary for 5.4.99.45 extracted from

  • Klassen, R.; Schaffrath, R.
    Role of pseudouridine formation by Deg1 for functionality of two glutamine isoacceptor tRNAs (2017), Biomolecules, 7, pii: E8 .
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
additional information the thermosensitive growth phenotype of deg1 mutants is specifically suppressible by overexpression of tRNAGln UUG but not tRNAGln CUG or any other tRNA carrying U38 or U39, which is modified by Deg1 to Psi, with clear suppression of deg1-induced thermosensitivity by multicopy tRNAGln UUG but not tRNAGln CUG or other tester tRNAs. Rescue by multicopy tRNAGln UUG is clearly incomplete as growth at 39°C is not fully restored to wild-type levels. Negative genetic interactions between DEG1, Elongator and URM1 can be extended to conditions of TORC1 inhibition, rapamycin sensitivity of deg1, urm1 and elp3 single and all possible double mutant combinations, overview. Deletion of the DEG1 gene in strain W303-1B and score of adenine auxotrophy as well as color colony in direct comparison with an elp3 mutant that is known to affect SUP4-mediated read-through Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tRNA uridine38/39 Saccharomyces cerevisiae
-
 tRNA pseudouridine38/39
-
 ?
tRNA uridine38/39 Saccharomyces cerevisiae ATCC 204508 / S288c / BY4741
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 tRNA pseudouridine38/39
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P31115
-
-
Saccharomyces cerevisiae ATCC 204508 / S288c / BY4741 P31115
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA uridine38/39
-
 Saccharomyces cerevisiae tRNA pseudouridine38/39
-
 ?
tRNA uridine38/39
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 Saccharomyces cerevisiae ATCC 204508 / S288c / BY4741 tRNA pseudouridine38/39
-
 ?

Synonyms

Synonyms Comment Organism
Deg1
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 Saccharomyces cerevisiae
PUS3
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 Saccharomyces cerevisiae
PUS3/DEG1
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 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction loss of Deg1/Pus3 and concomitant elimination of pseudouridine in tRNA at positions 38 and 39 (Psi38/39) specifically impairs the function of tRNAGln UUG under conditions of temperature-induced downregulation of wobble uridine thiolation in budding yeast. In the tRNATyr GPspiA variant SUP4, UAA read-through is enabled due to an anticodon mutation (UPsiA), whereas sup70-65 is a mutant form of tRNAGln CUG (SUP70) that mediates UAG decoding due to a mutation of the anticodon-loop closing base pair (G31:C39 to A31:C39). While SUP4 function is unaltered in deg1/pus3 mutants, the ability of sup70-65 to mediate non-sense suppression and to complement a genomic deletion of the essential SUP70 gene is severely compromised. Differential suppression of growth defects in deg1 mutants by multi-copy SUP70 or tQ(UUG). Phenotypic effects of DEG1 deletion may be rescuable by overexpression of tRNAGln UUG, since this is the major Gln isoacceptor and consequently more important for overall translation as compared to the minor tRNAGln CUG Saccharomyces cerevisiae
physiological function in yeast, Deg1 dependent formation of Psi occurs in a majority of tRNAs either at position 38 or 39. Psi38 is most important for tRNAGln UUG function under heat stress but becomes crucial for tRNAGln CUG as well when the anticodon loop is destabilized by the sup70-65 mutation. Thus, Psi38/39 may protect the anticodon loop configuration from disturbances by loss of other modifications or base changes. Deg1-mediated Psi39 in itself does not detectably impact on the function of SUP4 in non-sense suppression in two different read-through contexts. Role of Psi38/39 for non-sense suppression for SUP4 or SUP70-65 Saccharomyces cerevisiae