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Literature summary for 5.4.99.42 extracted from

  • Ansmant, I.; Motorin, Y.; Massenet, S.; Grosjean, H.; Branlant, C.
    Identification and characterization of the tRNA:Psi 31-synthase (Pus6p) of Saccharomyces cerevisiae (2001), J. Biol. Chem., 276, 34934-34940.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
recombinant His6-tagged Ygr169 protein produced in Escherichia coli is capable of forming pseudouridine31 in vitro using tRNAs extracted from the DELTAYGR169c yeast cells as substrates Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D168A mutation abolishes tRNA pseudouridine31 synthase activity Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Saccharomyces cerevisiae 5737
-
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tRNA uridine31 Saccharomyces cerevisiae the enzyme is responsible for modification of cytoplasmic and mitochondrial tRNAs at position 31 tRNA pseudouridine31
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P53294
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA uridine31 the enzyme is responsible for modification of cytoplasmic and mitochondrial tRNAs at position 31 Saccharomyces cerevisiae tRNA pseudouridine31
-
 ?
tRNA uridine31 cytoplasmic and mitochondrial tRNA(Met), mitochondrial tRNA(Lys) and tRNA(Ser). Recombinant His6-tagged Ygr169 protein produced in Escherichia coli is capable of forming pseudouridine31 in vitro using tRNAs extracted from the DELTAYGR169c yeast cells as substrates. The enzyme does not catalyse the pseudouridylation of uridine31 Saccharomyces cerevisiae tRNA pseudouridine31
-
 ?

Synonyms

Synonyms Comment Organism
pseudouridylate synthase 6
-
 Saccharomyces cerevisiae
Pus6p
-
 Saccharomyces cerevisiae
tRNA:Psi31-synthase
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction the deletion mutant strain DELTAYGR169c shows no detectable growth phenotype. The only difference in pseudouridine formation in stable cellular RNAs is the absence of pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs. Complementation of the DELTAYGR169c strain by a plasmid bearing the wild-type YGR169c ORF restores pseudouridine31 formation in tRNA Saccharomyces cerevisiae