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Literature summary for 5.4.99.4 extracted from
- Assay and purification of the adenosylcobalamin-dependent 2-methyleneglutarate mutase from Clostridium barkeri (1989), Eur. J. Biochem., 184, 103-107.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4 | - |
2-Methyleneglutarate | - |
Eubacterium barkeri |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
4 * 70000, SDS-PAGE | Eubacterium barkeri |
| 290000 | - |
gel filtration | Eubacterium barkeri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eubacterium barkeri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Eubacterium barkeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
continuous spectrophotometric assay | Eubacterium barkeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Methyleneglutarate | - |
Eubacterium barkeri | 2-Methylene-3-methylsuccinate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 70000, SDS-PAGE | Eubacterium barkeri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cobamide | dependent on | Eubacterium barkeri | |
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Release 2023.1 (January 2023)
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