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Literature summary for 5.4.99.34 extracted from

  • Andre, C.; Legay, S.; Deleruelle, A.; Nieuwenhuizen, N.; Punter, M.; Brendolise, C.; Cooney, J.; Lateur, M.; Hausman, J.; Larondelle, Y.; Laing, W.
    Multifunctional oxidosqualene cyclases and cytochrome P450 involved in the biosynthesis of apple fruit triterpenic acids (2016), New Phytol., 211, 1279-1294 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.34

Cloned(Commentary)

Cloned (Comment) Organism
gene OSC4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of germanicol, beta-amyrin, and lupeol Malus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene Malus domestica
-
 germanicol
-
 r
additional information Malus domestica although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level ?
-
 ?

Organism

Organism UniProt Comment Textmining
Malus domestica A0A142LT03
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Malus domestica
-
additional information although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level Malus domestica
-
plant epidermis
-
 Malus domestica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
 Malus domestica germanicol
-
 r
(S)-2,3-oxidosqualene via dammarenyl cation, low activity Malus domestica beta-amyrin
-
 r
(S)-2,3-oxidosqualene via dammarenyl cation, low activity Malus domestica lupeol
-
 r
additional information although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level Malus domestica ?
-
 ?
additional information gene MdOSC4 encodes a multifunctional oxidosqualene cyclase producing an oleanane-type triterpene, putatively identified as germanicol, as well as beta-amyrin and lupeol, in the proportion 82:14:4 Malus domestica ?
-
 ?

Synonyms

Synonyms Comment Organism
OCS4
-
 Malus domestica

General Information

General Information Comment Organism
evolution MdOSC4 contains the highly conserved DCTAE motif, which is implicated in substrate binding, and six repeats of the QW motifs, typical of the triterpene synthase superfamily, sequence comparisons and phylogenetic analysis Malus domestica
metabolism analysis of biosynthetic pathway of triterpenic acids in apple fruits, overview Malus domestica
additional information analysis of triterpenes from apple fruits in a collection of 20 contrasting apple cultivars, expression patterns of triterpene-related genes in different apple cultivars, overview Malus domestica
physiological function the enzyme is involved in the biosynthesis of pentacyclic triterpenes, which, in apple, account for 32-70% of the epicuticular waxes, depending on the cultivar Malus domestica