BRENDA - Enzyme Database show
show all sequences of 5.4.99.33

Recombinant yeast as a functional tool for understanding bitterness and cucurbitacin biosynthesis in watermelon (Citrullus spp.)

Davidovich-Rikanati, R.; Shalev, L.; Baranes, N.; Meir, A.; Itkin, M.; Cohen, S.; Zimbler, K.; Portnoy, V.; Ebizuka, Y.; Shibuya, M.; Burger, Y.; Katzir, N.; Schaffer, A.A.; Lewinsohn, E.; Tadmor, Y.; Yeast 32, 103-114 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CcCDS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression in Saccharomyces cerevisiae
Citrullus colocynthis
gene CpCDS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression in Saccharomyces cerevisiae
Cucurbita pepo
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
Cucurbita pepo
-
cucurbitadienol
-
-
?
(3S)-2,3-epoxy-2,3-dihydrosqualene
Citrullus colocynthis
-
cucurbitadienol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Citrullus colocynthis
A0A0D3QXV2
-
-
Cucurbita pepo
Q6BE24
-
-
no activity in Citrullus lanatus var. lanatus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Citrullus colocynthis
-
fruit
-
Cucurbita pepo
-
leaf
-
Citrullus colocynthis
-
root
-
Citrullus colocynthis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
749428
Cucurbita pepo
cucurbitadienol
-
-
-
?
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
749428
Citrullus colocynthis
cucurbitadienol
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CcCDS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression in Saccharomyces cerevisiae
Citrullus colocynthis
gene CpCDS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression in Saccharomyces cerevisiae
Cucurbita pepo
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
Cucurbita pepo
-
cucurbitadienol
-
-
?
(3S)-2,3-epoxy-2,3-dihydrosqualene
Citrullus colocynthis
-
cucurbitadienol
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Citrullus colocynthis
-
fruit
-
Cucurbita pepo
-
leaf
-
Citrullus colocynthis
-
root
-
Citrullus colocynthis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
749428
Cucurbita pepo
cucurbitadienol
-
-
-
?
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
749428
Citrullus colocynthis
cucurbitadienol
-
-
-
?
Other publictions for EC 5.4.99.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746042
Zhang
Oxidation of cucurbitadienol ...
Siraitia grosvenorii
Plant Cell Physiol.
57
1000-1007
2016
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1
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2
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1
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1
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2
2
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746038
Dai
Functional characterization o ...
Siraitia grosvenorii
Plant Cell Physiol.
56
1172-1182
2015
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2
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749428
Davidovich-Rikanati
Recombinant yeast as a functi ...
Citrullus colocynthis, Cucurbita pepo, no activity in Citrullus lanatus var. lanatus
Yeast
32
103-114
2015
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2
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9
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4
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2
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716949
Shibuya
-
Cucurbitadienol synthase, the ...
Cucurbita pepo
Tetrahedron
60
6995-7003
2004
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1
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3
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1
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1
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1
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1
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