Cloned (Comment) | Organism |
---|---|
gene CBS, DNA and amino acid sequence determination and analysis, digital gene expression (DGE) profile analysis, sequence comparisons and phylogenetic analysis of codon-optimized gene, SgCbQ is functionally expressed in the lanosterol synthase-deficient Saccharomyces cerevisiae strain GIL77 leading to accumulation of cucurbitadienol as the sole product | Siraitia grosvenorii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene | Siraitia grosvenorii | - |
cucurbitadienol | - |
? | |
additional information | Siraitia grosvenorii | GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Siraitia grosvenorii | K7NBZ9 | cultivated in Guangxi Province of China | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | expression profiles of SgCbQ gene during the three important stages of fruit development | Siraitia grosvenorii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Siraitia grosvenorii | cucurbitadienol | - |
? | |
additional information | GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol | Siraitia grosvenorii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CBS | - |
Siraitia grosvenorii |
SgCbQ | - |
Siraitia grosvenorii |
General Information | Comment | Organism |
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evolution | SgCbQ clusters with the other three functionally characterized cucurbitadienol synthases and forms a new subfamily that lies between the branches of cycloartenol synthase and lanosterol synthase, non-rooted phylogenetic tree analysis, overview | Siraitia grosvenorii |
metabolism | cucurbitadienol synthase and triterpene glycosyltransferase are involved in biosynthesis of mogrosides in Siraitia grosvenorii. Mogrosides, the major bioactive components isolated from the fruits of Siraitia grosvenorii, are a family of cucurbitane-type tetracyclic triterpenoid saponins that are used worldwide as high-potency sweeteners and possess a variety of notable pharmacological activities. Mogrosides are synthesized from 2,3-oxidosqualene via a series of reactions catalyzed by cucurbitadienol synthase (CbQ), Cyt P450s (P450s), and UDP glycosyltransferases (UGTs) in vivo, proposed biosynthetic pathway of mogrosides, overview | Siraitia grosvenorii |