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show all sequences of 5.4.99.33

Functional characterization of cucurbitadienol synthase and triterpene glycosyltransferase involved in biosynthesis of mogrosides from Siraitia grosvenorii

Dai, L.; Liu, C.; Zhu, Y.; Zhang, J.; Men, Y.; Zeng, Y.; Sun, Y.; Plant Cell Physiol. 56, 1172-1182 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CBS, DNA and amino acid sequence determination and analysis, digital gene expression (DGE) profile analysis, sequence comparisons and phylogenetic analysis of codon-optimized gene, SgCbQ is functionally expressed in the lanosterol synthase-deficient Saccharomyces cerevisiae strain GIL77 leading to accumulation of cucurbitadienol as the sole product
Siraitia grosvenorii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
Siraitia grosvenorii
-
cucurbitadienol
-
-
?
additional information
Siraitia grosvenorii
GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Siraitia grosvenorii
K7NBZ9
cultivated in Guangxi Province of China
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
expression profiles of SgCbQ gene during the three important stages of fruit development
Siraitia grosvenorii
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
746038
Siraitia grosvenorii
cucurbitadienol
-
-
-
?
additional information
GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol
746038
Siraitia grosvenorii
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CBS, DNA and amino acid sequence determination and analysis, digital gene expression (DGE) profile analysis, sequence comparisons and phylogenetic analysis of codon-optimized gene, SgCbQ is functionally expressed in the lanosterol synthase-deficient Saccharomyces cerevisiae strain GIL77 leading to accumulation of cucurbitadienol as the sole product
Siraitia grosvenorii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
Siraitia grosvenorii
-
cucurbitadienol
-
-
?
additional information
Siraitia grosvenorii
GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
expression profiles of SgCbQ gene during the three important stages of fruit development
Siraitia grosvenorii
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
746038
Siraitia grosvenorii
cucurbitadienol
-
-
-
?
additional information
GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol
746038
Siraitia grosvenorii
?
-
-
-
-
General Information
General Information
Commentary
Organism
evolution
SgCbQ clusters with the other three functionally characterized cucurbitadienol synthases and forms a new subfamily that lies between the branches of cycloartenol synthase and lanosterol synthase, non-rooted phylogenetic tree analysis, overview
Siraitia grosvenorii
metabolism
cucurbitadienol synthase and triterpene glycosyltransferase are involved in biosynthesis of mogrosides in Siraitia grosvenorii. Mogrosides, the major bioactive components isolated from the fruits of Siraitia grosvenorii, are a family of cucurbitane-type tetracyclic triterpenoid saponins that are used worldwide as high-potency sweeteners and possess a variety of notable pharmacological activities. Mogrosides are synthesized from 2,3-oxidosqualene via a series of reactions catalyzed by cucurbitadienol synthase (CbQ), Cyt P450s (P450s), and UDP glycosyltransferases (UGTs) in vivo, proposed biosynthetic pathway of mogrosides, overview
Siraitia grosvenorii
General Information (protein specific)
General Information
Commentary
Organism
evolution
SgCbQ clusters with the other three functionally characterized cucurbitadienol synthases and forms a new subfamily that lies between the branches of cycloartenol synthase and lanosterol synthase, non-rooted phylogenetic tree analysis, overview
Siraitia grosvenorii
metabolism
cucurbitadienol synthase and triterpene glycosyltransferase are involved in biosynthesis of mogrosides in Siraitia grosvenorii. Mogrosides, the major bioactive components isolated from the fruits of Siraitia grosvenorii, are a family of cucurbitane-type tetracyclic triterpenoid saponins that are used worldwide as high-potency sweeteners and possess a variety of notable pharmacological activities. Mogrosides are synthesized from 2,3-oxidosqualene via a series of reactions catalyzed by cucurbitadienol synthase (CbQ), Cyt P450s (P450s), and UDP glycosyltransferases (UGTs) in vivo, proposed biosynthetic pathway of mogrosides, overview
Siraitia grosvenorii
Other publictions for EC 5.4.99.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746042
Zhang
Oxidation of cucurbitadienol ...
Siraitia grosvenorii
Plant Cell Physiol.
57
1000-1007
2016
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746038
Dai
Functional characterization o ...
Siraitia grosvenorii
Plant Cell Physiol.
56
1172-1182
2015
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749428
Davidovich-Rikanati
Recombinant yeast as a functi ...
Citrullus colocynthis, Cucurbita pepo, no activity in Citrullus lanatus var. lanatus
Yeast
32
103-114
2015
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716949
Shibuya
-
Cucurbitadienol synthase, the ...
Cucurbita pepo
Tetrahedron
60
6995-7003
2004
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