Literature summary for 5.4.99.31 extracted from

Loeschcke, A.; Dienst, D.; Wewer, V.; Hage-Huelsmann, J.; Dietsch, M.; Kranz-Finger, S.; Hueren, V.; Metzger, S.; Urlacher, V.B.; Gigolashvili, T.; Kopriva, S.; Axmann, I.M.; Drepper, T.; Jaeger, K.E.
The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis sp. PCC 6803 as new hosts for cyclic plant triterpene biosynthesis (2017), PLoS ONE, 12, e0189816 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene THAS1, recombinant expression in Rhodobacter capsulatus strain SB1003. and Synechocystis sp. PCC 6803 using promoters Pnif and PcoaT, coexpression with CAS1, LUP1, and MRN1. Subcloning in Saccharomyces cerevisiae strain GIL77. Isolation of triterpenes, while cycloartenol and lupeol represent typical plant triterpenes with tetracyclic plant sterol and pentacyclic plant triterpene scaffolds, respectively, thalianol and marneral exhibit more unusual tri- and monocyclic structures	Arabidopsis thaliana

Cloned (Comment)

Organism

gene THAS1, recombinant expression in Rhodobacter capsulatus strain SB1003. and Synechocystis sp. PCC 6803 using promoters Pnif and PcoaT, coexpression with CAS1, LUP1, and MRN1. Subcloning in Saccharomyces cerevisiae strain GIL77. Isolation of triterpenes, while cycloartenol and lupeol represent typical plant triterpenes with tetracyclic plant sterol and pentacyclic plant triterpene scaffolds, respectively, thalianol and marneral exhibit more unusual tri- and monocyclic structures

Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	2,3-oxidosqualene production is implemented and subsequently combined with different cyclization reactions catalyzed by the representative oxidosqualene cyclases CAS1 (cycloartenol synthase), LUP1 (lupeol synthase), THAS1 (thalianol synthase) and MRN1 (marneral synthase), derived from model plant Arabidopsis thaliana, in the two alternative hosts for biosynthesis of cyclic plant triterpenes, the metabolically versatile photosynthetic alpha-proteobacterium Rhodobacter capsulatus strain SB1003 and cyanobacterium Synechocystis sp. PCC 6803, triterpene pathway design, overview. While successful accumulation of 2,3-oxidosqualene can be detected by LC-MS analysis in both hosts, cyclase expression results in differential production profiles. CAS1 catalyzes conversion to only cycloartenol, but expression of LUP1 yields lupeol and a triterpenoid matching an oxidation product of lupeol, in both hosts. In contrast, THAS1 expression does not lead to cyclic product formation in either host, whereas MRN1-dependent production of marnerol and hydroxymarnerol is observed in Synechocystis but not in Rhodobacter capsulatus. 2,3-Oxidosqualene cyclization in heterologous phototrophic bacteria is basically feasible but efficient conversion depends on both the respective cyclase enzyme and individual host properties	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene	Arabidopsis thaliana	-	thalianol	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9FI37	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene	-	Arabidopsis thaliana	thalianol	-	?

Synonyms

Synonyms	Comment	Organism
At5g48010	locus name	Arabidopsis thaliana
THAS1	-	Arabidopsis thaliana