Cloned (Comment) | Organism |
---|---|
gene UAM3 or RGP3, OsUAM3 expression is independent from OsUAM1 and OsUAM2 co-expression networks | Oryza sativa Japonica Group |
Protein Variants | Comment | Organism |
---|---|---|
additional information | RNA interference (RNAi)-knockdown transformants (OsUAM3-KD) specific for OsUAM3. Modifications of cell wall polysaccharides at the cellular level are determined using antibodies against polysaccharides including Araf, low levels of arabinan in OsUAM3-KD pollen grains | Oryza sativa Japonica Group |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-beta-L-arabinofuranose | Oryza sativa Japonica Group | - |
UDP-beta-L-arabinopyranose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Japonica Group | Q6Z4G3 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | OsUAM3 coexpression networks include pectin catabolic enzymes. Co-expression network in silico analysis, quantitative RT-PCR analysis of OsUAM isozymes gene expression in various organs at different developmental stages, overview | Oryza sativa Japonica Group | - |
pollen | OsUAM1 and OsUAM2 are expressed ubiquitously throughout plant development, but OsUAM3 is expressed primarily in reproductive tissue, particularly at the pollen cell wall formation developmental stage | Oryza sativa Japonica Group | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-beta-L-arabinofuranose | - |
Oryza sativa Japonica Group | UDP-beta-L-arabinopyranose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
OsUAM3 | - |
Oryza sativa Japonica Group |
UDP-arabinopyranose mutase 3 | - |
Oryza sativa Japonica Group |
General Information | Comment | Organism |
---|---|---|
evolution | the UAM gene family has three members in Oryza sativa | Oryza sativa Japonica Group |
malfunction | OsUAM3-knockdown plants grow normally, but show abnormal phenotypes in reproductive tissues, especially in terms of the pollen cell wall and exine. Pollen formation is abnormal in OsUAM3-KD plants, and KI-I2 staining for starch content shows that OsUAM3-KD pollen is abnormal and accumulates little starch. Low levels of arabinan in OsUAM3-KD pollen grains | Oryza sativa Japonica Group |
physiological function | isozyme OsUAM3 has a different function from that of isozymes OsUAM1 and OsUAM2i, sozyme UDP-arabinopyranose mutase 3 is required for pollen wall morphogenesis in Oryza sativa. L-Arabinose is one of the main constituents of cell wall polysaccharides such as pectic rhamnogalacturonan I, RG-I, glucuronoarabinoxylans and other glycoproteins. It is found predominantly in the furanose form rather than in the thermodynamically more stable pyranose form. UDP-L-arabinofuranose (UDP-Araf), rather than UDP-L-arabinopyranose (UDP-Arap), is a sugar donor for the biosynthesis of arabinofuranosyl (Araf) residues. UDP-arabinopyranose mutases (UAMs) interconvert UDP-Araf and UDP-Arap and are involved in the biosynthesis of polysaccharides including Araf | Oryza sativa Japonica Group |