Cloned (Comment) | Organism |
---|---|
isozyme RGP1, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 transformation method, quantitative real-time PCR enzyme expression analysis | Brachypodium distachyon |
isozyme RGP2, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 method, quantitative real-time PCR enzyme expression analysis | Brachypodium distachyon |
isozyme RGP3, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 transformation method, quantitative real-time PCR enzyme expression analysis | Brachypodium distachyon |
isozyme RGP4, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 transformation method, quantitative real-time PCR enzyme expression analysis | Brachypodium distachyon |
Protein Variants | Comment | Organism |
---|---|---|
D110A | site-directed mutagenesis | Brachypodium distachyon |
D111A | site-directed mutagenesis | Brachypodium distachyon |
D112A | site-directed mutagenesis | Brachypodium distachyon |
H273A | site-directed mutagenesis | Brachypodium distachyon |
additional information | specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature | Brachypodium distachyon |
additional information | specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature. Targeted manipulation of UDP-sugar biosynthesis can result in biomass with substantially altered compositions | Brachypodium distachyon |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-beta-L-arabinofuranose | Brachypodium distachyon | - |
UDP-beta-L-arabinopyranose | - |
? | |
UDP-beta-L-arabinofuranose | Brachypodium distachyon Bd21 | - |
UDP-beta-L-arabinopyranose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brachypodium distachyon | I1GQE9 | - |
- |
Brachypodium distachyon | I1GSJ4 | - |
- |
Brachypodium distachyon | I1HRV4 | - |
- |
Brachypodium distachyon | I1J2W9 | - |
- |
Brachypodium distachyon Bd21 | I1J2W9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme is a reversibly glycosylated protein | Brachypodium distachyon |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Brachypodium distachyon | - |
leaf sheath | - |
Brachypodium distachyon | - |
additional information | isozyme RGP3 expression is present in sheath and stem samples but not leaf blade samples regardless of developmental stage, RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview. RGP3 shows a low expression compared to the other isozymes | Brachypodium distachyon | - |
additional information | RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview | Brachypodium distachyon | - |
seedling | - |
Brachypodium distachyon | - |
stem | - |
Brachypodium distachyon | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-beta-L-arabinofuranose | - |
Brachypodium distachyon | UDP-beta-L-arabinopyranose | - |
? | |
UDP-beta-L-arabinofuranose | - |
Brachypodium distachyon Bd21 | UDP-beta-L-arabinopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BdRGP1 | - |
Brachypodium distachyon |
Bradi1g15050 | locus name | Brachypodium distachyon |
Bradi1g21990 | locus name | Brachypodium distachyon |
Bradi2g50660 | locus name | Brachypodium distachyon |
Bradi5g24850 | locus name | Brachypodium distachyon |
LOC100822188 | locus name | Brachypodium distachyon |
LOC100828998 | locus name | Brachypodium distachyon |
LOC100836426 | locus name | Brachypodium distachyon |
LOC100838265 | locus name | Brachypodium distachyon |
RGP1 | - |
Brachypodium distachyon |
RGP2 | - |
Brachypodium distachyon |
RGP3 | - |
Brachypodium distachyon |
RGP4 | - |
Brachypodium distachyon |
General Information | Comment | Organism |
---|---|---|
malfunction | RNAi mutants of isozyme BdRGP1 result in large alterations in cell wall carbohydrate composition with significant decreases in cell wall arabinose content but with minimal change in plant stature. Real-time PCR analysis indicates that gene expression levels for BdRGP1, BdRGP2, and BdRGP3 are reduced in RNAi-RGP1 plants to 15-20%of controls. Cell wall arabinose content is reduced by 23-51%of control levels. No alterations in cell wall xylose and glucose content are observed. Corresponding decreases in cell wall ferulic acid and ferulic acid-dimers are observed. Additionally, cell wall 4-coumarates are decreased. The cell wall decrease in 4-coumarates corresponds to arabinose-coupled 4-coumarates. Xylanase-mediated digestibility of RNAi-RGP1 Brachypodium cell walls results in a near 2fold increase of released total carbohydrate. Cellulolytic hydrolysis of cell wall material is inhibited in leaves of RNAi-RGP1 mutants | Brachypodium distachyon |
physiological function | the enzyme is involved in cell wall carbohydrate biosynthesis, the cell wall composition has a complex effect on digestibility of grass plants | Brachypodium distachyon |