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Literature summary for 5.4.99.30 extracted from

  • Rancour, D.M.; Hatfield, R.D.; Marita, J.M.; Rohr, N.A.; Schmitz, R.J.
    Cell wall composition and digestibility alterations in Brachypodium distachyon achieved through reduced expression of the UDP-arabinopyranose mutase (2015), Front. Plant Sci., 6, 446 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.30

Cloned(Commentary)

Cloned (Comment) Organism
isozyme RGP1, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 transformation method, quantitative real-time PCR enzyme expression analysis Brachypodium distachyon
isozyme RGP2, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 method, quantitative real-time PCR enzyme expression analysis Brachypodium distachyon
isozyme RGP3, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 transformation method, quantitative real-time PCR enzyme expression analysis Brachypodium distachyon
isozyme RGP4, recombinant overexpression in Brachypodium distachyon strain Bd21-3 using the Agrobacterum tumefaciens strain AGL1 transformation method, quantitative real-time PCR enzyme expression analysis Brachypodium distachyon

Protein Variants

Protein Variants Comment Organism
D110A site-directed mutagenesis Brachypodium distachyon
D111A site-directed mutagenesis Brachypodium distachyon
D112A site-directed mutagenesis Brachypodium distachyon
H273A site-directed mutagenesis Brachypodium distachyon
additional information specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature Brachypodium distachyon
additional information specific knockout of the isozyme by RNAi-mediated gene-suppression, overexpression by constitutive expression approach, phenotypes, phenotypes, detailed overview. Analysis of phenotypes for generation of UDP-sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in cell wall carbohydrate composition changes with improved digestibility and normal plant stature. Targeted manipulation of UDP-sugar biosynthesis can result in biomass with substantially altered compositions Brachypodium distachyon

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-beta-L-arabinofuranose Brachypodium distachyon
-
 UDP-beta-L-arabinopyranose
-
 ?
UDP-beta-L-arabinofuranose Brachypodium distachyon Bd21
-
 UDP-beta-L-arabinopyranose
-
 ?

Organism

Organism UniProt Comment Textmining
Brachypodium distachyon I1GQE9
-
-
Brachypodium distachyon I1GSJ4
-
-
Brachypodium distachyon I1HRV4
-
-
Brachypodium distachyon I1J2W9
-
-
Brachypodium distachyon Bd21 I1J2W9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme is a reversibly glycosylated protein Brachypodium distachyon

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Brachypodium distachyon
-
leaf sheath
-
 Brachypodium distachyon
-
additional information isozyme RGP3 expression is present in sheath and stem samples but not leaf blade samples regardless of developmental stage, RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview. RGP3 shows a low expression compared to the other isozymes Brachypodium distachyon
-
additional information RGP isozymes expression patterns in plant tissues, quantitative real-time PCR enzyme expression analysis, overview Brachypodium distachyon
-
seedling
-
 Brachypodium distachyon
-
stem
-
 Brachypodium distachyon
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-beta-L-arabinofuranose
-
 Brachypodium distachyon UDP-beta-L-arabinopyranose
-
 ?
UDP-beta-L-arabinofuranose
-
 Brachypodium distachyon Bd21 UDP-beta-L-arabinopyranose
-
 ?

Synonyms

Synonyms Comment Organism
BdRGP1
-
 Brachypodium distachyon
Bradi1g15050 locus name Brachypodium distachyon
Bradi1g21990 locus name Brachypodium distachyon
Bradi2g50660 locus name Brachypodium distachyon
Bradi5g24850 locus name Brachypodium distachyon
LOC100822188 locus name Brachypodium distachyon
LOC100828998 locus name Brachypodium distachyon
LOC100836426 locus name Brachypodium distachyon
LOC100838265 locus name Brachypodium distachyon
RGP1
-
 Brachypodium distachyon
RGP2
-
 Brachypodium distachyon
RGP3
-
 Brachypodium distachyon
RGP4
-
 Brachypodium distachyon

General Information

General Information Comment Organism
malfunction RNAi mutants of isozyme BdRGP1 result in large alterations in cell wall carbohydrate composition with significant decreases in cell wall arabinose content but with minimal change in plant stature. Real-time PCR analysis indicates that gene expression levels for BdRGP1, BdRGP2, and BdRGP3 are reduced in RNAi-RGP1 plants to 15-20%of controls. Cell wall arabinose content is reduced by 23-51%of control levels. No alterations in cell wall xylose and glucose content are observed. Corresponding decreases in cell wall ferulic acid and ferulic acid-dimers are observed. Additionally, cell wall 4-coumarates are decreased. The cell wall decrease in 4-coumarates corresponds to arabinose-coupled 4-coumarates. Xylanase-mediated digestibility of RNAi-RGP1 Brachypodium cell walls results in a near 2fold increase of released total carbohydrate. Cellulolytic hydrolysis of cell wall material is inhibited in leaves of RNAi-RGP1 mutants Brachypodium distachyon
physiological function the enzyme is involved in cell wall carbohydrate biosynthesis, the cell wall composition has a complex effect on digestibility of grass plants Brachypodium distachyon