Cloned (Comment) | Organism |
---|---|
gene RGP1, recombinant overexpression of His-MBP-tagged isozyme in Escherichia coli strain BL21 Gold | Arabidopsis thaliana |
gene RGP2, recombinant overexpression of His-MBP-tagged isozyme in Escherichia coli strain BL21 Gold | Arabidopsis thaliana |
gene RGP3, recombinant overexpression of His-MBP-tagged isozyme in Escherichia coli strain BL21 Gold | Arabidopsis thaliana |
isozyme UAM1, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 | Oryza sativa Japonica Group |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates, best divalent metal ion, optimal at 0.08 mM, about 20% of maximal activity at 1 mM, complete inhibition at 5 mM; activates, best divalent metal ion, optimal at 0.08 mM, about 20% of maximal activity at 1 mM, complete inhibition at 5 mM; activates, best divalent metal ion, optimal at 0.08 mM, about 25% of maximal activity at 1 mM, complete inhibition at 5 mM | Arabidopsis thaliana | |
n-butanol | - |
Arabidopsis thaliana | |
n-butanol | - |
Oryza sativa Japonica Group |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | plant cell wall and leaf cell wall | Arabidopsis thaliana | 5618 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Ca2+ | Arabidopsis thaliana | |
Ca2+ | displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Ca2+ | Oryza sativa Japonica Group | |
Ca2+ | displays less than 5% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Ca2+ | Arabidopsis thaliana | |
Co2+ | displays about 60% of the activity with Mn2+ at 0.08 mM | Arabidopsis thaliana | |
Co2+ | displays about 60% of the activity with Mn2+ at 0.08 mM | Oryza sativa Japonica Group | |
Co2+ | displays about 65% of the activity with Mn2+ at 0.08 mM | Arabidopsis thaliana | |
Cu2+ | displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Cu2+ | Arabidopsis thaliana | |
Cu2+ | displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Cu2+ | Oryza sativa Japonica Group | |
Cu2+ | displays less than 5% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Cu2+ | Arabidopsis thaliana | |
Mg2+ | displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Mg2+ | Arabidopsis thaliana | |
Mg2+ | displays less than 10% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Mg2+ | Oryza sativa Japonica Group | |
Mg2+ | displays less than 5% of the activity with Mn2+ at 0.08 mM, the activity does not increase at higher concentrations of Mg2+ | Arabidopsis thaliana | |
Mn2+ | activates, best divalent metal ion, optimal at 0.08 mM, 45% of maximal activity at 5 mM | Oryza sativa Japonica Group | |
Mn2+ | activates, best divalent metal ion, optimal at 0.08 mM, about 20% of maximal activity at 1 mM, complete inhibition at 5 mM | Arabidopsis thaliana | |
Mn2+ | activates, best divalent metal ion, optimal at 0.08 mM, about 25% of maximal activity at 1 mM, complete inhibition at 5 mM | Arabidopsis thaliana | |
additional information | the enzyme requires divalent metal ions for catalytic activity, divalent metal ion dependency of UAM from Arabidopsis thaliana, compared to Oryza sativa | Arabidopsis thaliana | |
additional information | the enzyme requires divalent metal ions for catalytic activity, divalent metal ion dependency of UAM from Oryza sativa, compared to Arabidopsis thaliana | Oryza sativa Japonica Group | |
Zn2+ | displays about 60% of the activity with Mn2+ at 0.08 mM | Arabidopsis thaliana | |
Zn2+ | displays about 70% of the activity with Mn2+ at 0.08 mM | Arabidopsis thaliana | |
Zn2+ | displays about 70% of the activity with Mn2+ at 0.08 mM | Oryza sativa Japonica Group |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-beta-L-arabinofuranose | Arabidopsis thaliana | - |
UDP-beta-L-arabinopyranose | - |
? | |
UDP-beta-L-arabinofuranose | Oryza sativa Japonica Group | - |
UDP-beta-L-arabinopyranose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O22666 | - |
- |
Arabidopsis thaliana | Q9LFW1 | - |
- |
Arabidopsis thaliana | Q9SRT9 | - |
- |
Oryza sativa Japonica Group | Q8H8T0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-MBP-tagged isozyme RGP1 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration | Arabidopsis thaliana |
recombinant His-MBP-tagged isozyme RGP2 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration | Arabidopsis thaliana |
recombinant His-MBP-tagged isozyme RGP3 from Escherichia coli strain BL21 Gold by nickel affinity chromatography, tag cleavage with TEV protease, followed by another step of nickel affinity chromatography, and gel filtration | Arabidopsis thaliana |
recombinant His-tagged wild-type and mutant isozymes UAM1 from Escherichia coli strain BL21 by nickel affinity chromatography | Oryza sativa Japonica Group |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
additional information | some of the AtRGPs such as the more temperature stable AtRGP2 and tRGP3 may be expressed by the plant as a response to heat stress, or during different stages of development | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-beta-L-arabinofuranose | - |
Arabidopsis thaliana | UDP-beta-L-arabinopyranose | - |
? | |
UDP-beta-L-arabinofuranose | - |
Oryza sativa Japonica Group | UDP-beta-L-arabinopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtRGP1 | - |
Arabidopsis thaliana |
AtRGP2 | - |
Arabidopsis thaliana |
AtRGP3 | - |
Arabidopsis thaliana |
OsUAM1 | - |
Oryza sativa Japonica Group |
RGP1 | - |
Arabidopsis thaliana |
RGP2 | - |
Arabidopsis thaliana |
RGP3 | - |
Arabidopsis thaliana |
UAM | - |
Oryza sativa Japonica Group |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryza sativa Japonica Group |
55 | - |
broad optimum | Arabidopsis thaliana |
60 | - |
- |
Arabidopsis thaliana |
60 | - |
broad optimum | Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 100 | over 90% of maximal activity at 40-90°C, 60% at 20°C and 100°C, profile overview | Arabidopsis thaliana |
20 | 90 | over 50% of maximal activity within this range, profile overview | Arabidopsis thaliana |
30 | 95 | over 80% of maximal activity within this range, 40% at 15°C, over 90% at 35-85°C, profile overview | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Arabidopsis thaliana |
6.8 | - |
assay at | Oryza sativa Japonica Group |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | activity range, profile overview | Arabidopsis thaliana |
4.5 | 9 | activity range, profile overview | Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | some of the AtRGPs such as the more temperature stable AtRGP2 and tRGP3 may be expressed by the plant as a response to heat stress, or during different stages of development | up |
General Information | Comment | Organism |
---|---|---|
additional information | three-dimensional structure modeling of isozyme UAM1 | Oryza sativa Japonica Group |