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Literature summary for 5.4.99.29 extracted from
- A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe) (1995), RNA, 1, 437-448.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24432 | - |
x * 24432, calculated from sequence | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0AA37 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 23S rRNA uridine746 | the enzyme reacts specifically with uridine746 in 23S rRNA. The enzyme is also responsible for the formation of pseudouridine32 in tRNA | Escherichia coli | 23S rRNA pseudouridine746 | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24432, calculated from sequence | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 23S RNA PSI746 synthase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
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Release 2023.1 (January 2023)
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