Literature summary for 5.4.99.28 extracted from

Hamilton, C.S.; Greco, T.M.; Vizthum, C.A.; Ginter, J.M.; Johnston, M.V.; Mueller, E.G.
Mechanistic investigations of the pseudouridine synthase RluA using RNA containing 5-fluorouridine (2006), Biochemistry, 45, 12029-12038.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000308	-	tRNA uridine32	pH 7.5, 37°C, anticodon stem-loop of Escherichia coli tRNAPhe (17-mer)	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0AA37	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
tRNA uridine32 = tRNA pseudouridine32	mechanistic investigations	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
tRNA uridine32	stem-loop RNAs are good substrates for RluA	Escherichia coli	tRNA pseudouridine32	-	?

Synonyms

Synonyms	Comment	Organism
pseudouridine synthase RluA	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.068	-	tRNA uridine32	pH 7.5, 37°C, anticodon stem-loop of Escherichia coli tRNAPhe (17-mer)	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
220	-	tRNA uridine32	pH 7.5, 37°C, anticodon stem-loop of Escherichia coli tRNAPhe (17-mer)	Escherichia coli

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Release 2023.1 (January 2023)