Literature summary for 5.4.99.25 extracted from

Muller, S.; Fourmann, J.B.; Loegler, C.; Charpentier, B.; Branlant, C.
Identification of determinants in the protein partners aCBF5 and aNOP10 necessary for the tRNA:Psi55-synthase and RNA-guided RNA:PSI-synthase activities (2007), Nucleic Acids Res., 35, 5610-5624.

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Activating Compound

Activating Compound	Comment	Organism	Structure
protein aNOP10	interaction with the pseudouridine synthase aCBF5 is required for the RNA-guided RNA:pseudouridine-synthase activity. The stable anchoring of aCBF5 to tRNAs relies on its PUA domain and the tRNA CCA sequence. Nonetheless, interaction of aNOP10 with aCBF5 can counterbalance the absence of the PUA domain or the CCA sequence and more generally helps the aCBF5 tRNA:pseudouridine55-synthase activity. Substitution of the aNOP10 residue Y14 by an alanine disturbs this activity, it only impairs mildly the RNA-guided activity. The opposite effect is observed for the aNOP10 variant H31A	Pyrococcus abyssi

Protein Variants

Protein Variants	Comment	Organism
K53A	substitution K53A or R202A in aCBF5 impairs both the tRNA:pseudouridine55-synthase and the RNA-guided RNA:pseudouridine-synthase activities	Pyrococcus abyssi
R202A	substitution K53A or R202A in aCBF5 impairs both the tRNA:pseudouridine55-synthase and the RNA-guided RNA:pseudouridine-synthase activities	Pyrococcus abyssi

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus abyssi	Q9V1A5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
tRNA uridine55	the stable anchoring of aCBF5 to tRNAs relies on its PUA domain and the tRNA CCA sequence	Pyrococcus abyssi	tRNA pseudouridine55	-	?

Synonyms

Synonyms	Comment	Organism
aCBF5	-	Pyrococcus abyssi
tRNA:PSI55-synthase	-	Pyrococcus abyssi

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Release 2023.1 (January 2023)