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Literature summary for 5.4.99.25 extracted from

  • Roovers, M.; Hale, C.; Tricot, C.; Terns, M.P.; Terns, R.M.; Grosjean, H.; Droogmans, L.
    Formation of the conserved pseudouridine at position 55 in archaeal tRNA (2006), Nucleic Acids Res., 34, 4293-4301.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.25

Activating Compound

Activating Compound Comment Organism Structure
additional information pfuCbf5 can modify tRNA uridine55, but not rRNA, in the absence of Gar1, Nop10 and L7Ae. The activity of pfuCbf5 toward tRNA uridine55 is enhanced in the presence of the accessory proteins, suggesting that a multi-protein Cbf5 complex may function in pseudouridine55 synthesis in Pyrococcus furiosus Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA uridine55 assay with T7-transcribed pfutRNAAsp. Two distinct pseudouridine synthases specifically modify uridine55 in tRNA in vitro: 1. Cbf5, a protein known to play a role in RNA-guided modification of rRNA, and 2. pfuPus10 is not a member of the TruB/Pus4/Cbf5 family of pseudouridine synthases. Pus10 can pseudouridylate a truncated tRNA substrate and a tRNA lacking the 3'CCA. Cbf5 functions only on the full-length tRNA substrate including the 3'CCA end Pyrococcus furiosus tRNA pseudouridine55
-
 ?

Synonyms

Synonyms Comment Organism
Cbf5
-
 Pyrococcus furiosus
pfuCbf5
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 about 50% of maximal activity at 50°C and at 80°C, pseudouridine synthase Cbf5 Pyrococcus furiosus

General Information

General Information Comment Organism
evolution the finding that in archaea Cbf5 may function in tRNA uridine55 pseudouridylation as well as RNA-guided pseudouridylation of rRNA supports the idea that Cbf5 is a direct descendant of a primordial TruB/Pus4-like tRNA PSI synthase Pyrococcus furiosus