Crystallization (Comment) | Organism |
---|---|
vapor-phase diffusion method, crystal structure of TruB apoenzyme in the absence of RNA. Comparison of the TruB apoenzyme from Escherichia coli and the RNA-bound forms of Thermotoga maritima TruB provides insight into the structural basis for RNA recognition and specificity | Escherichia coli |
vapor-phase diffusion method, crystal structure of TruB in complex with 17-base stem-loop of RNA. Comparison of the TruB apoenzyme from Escherichia coli and the RNA-bound form of Thermotoga maritima TruB provides insight into the structural basis for RNA recognition and specificity | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P60340 | - |
- |
Thermotoga maritima | Q9WZW0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Thermotoga maritima |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
tRNA uridine55 = tRNA pseudouridine55 | this enzyme undergoes significant conformational changes on binding to its substrate. These conformational changes include the ordering of the thumb loop, which binds right into the RNA hairpin loop, and a 10° hinge movement of the C-terminal domain. TruB recognizes its RNA substrate through a combination of rigid docking and induced fit, with TruB first rigidly binding to its target and then maximizing the interaction by induced fit | Escherichia coli | |
tRNA uridine55 = tRNA pseudouridine55 | this enzyme undergoes significant conformational changes on binding to its substrate. These conformational changes include the ordering of the thumb loop, which binds right into the RNA hairpin loop, and a 10° hinge movement of the C-terminal domain. TruB recognizes its RNA substrate through a combination of rigid docking and induced fit, with TruB first rigidly binding to its target and then maximizing the interaction by induced fit | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
RNA pseudouridine synthase TruB | - |
Escherichia coli |
RNA pseudouridine synthase TruB | - |
Thermotoga maritima |
TruB | - |
Escherichia coli |
TruB | - |
Thermotoga maritima |