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Literature summary for 5.4.99.23 extracted from

  • Del Campo, M.; Ofengand, J.; Malhotra, A.
    Crystal structure of the catalytic domain of RluD, the only rRNA pseudouridine synthase required for normal growth of Escherichia coli (2004), RNA, 10, 231-239.
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
2.0 A structure of the catalytic domain of RluD (residues 77–326). The catalytic domain folds into a mainly antiparallel beta-sheet flanked by several loops and helices. A positively charged cleft that presumably binds RNA leads to the conserved Asp139. The RluD N-terminal S4 domain, connected by a flexible linker, is disordered in our structure. RluD is very similar in both catalytic domain structure and active site arrangement to the pseudouridine synthases RsuA, TruB, and TruA Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
23S rRNA uridine1911/uridine1915/uridine1917 Escherichia coli
-
 23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P33643
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
23S rRNA uridine1911/uridine1915/uridine1917
-
 Escherichia coli 23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917
-
 ?

Synonyms

Synonyms Comment Organism
RluD
-
 Escherichia coli

General Information

General Information Comment Organism
evolution the structure of RluD (a RluA family member) emphasizes that the RluA, RsuA, TruB, and TruA families of pseudouridine synthases arose by divergent evolution from a common ancestor Escherichia coli