Literature summary for 5.4.99.21 extracted from

Sunita, S.; Zhenxing, H.; Swaathi, J.; Cygler, M.; Matte, A.; Sivaraman, J.
Domain organization and crystal structure of the catalytic domain of E.coli RluF, a pseudouridine synthase that acts on 23S rRNA (2006), J. Mol. Biol., 359, 998-1009.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor-diffusion method, domain architecture and crystal structure of the catalytic domain of RluF at 2.6 A resolution. Limited proteolysis, mass spectrometry and N-terminal sequencing indicate that RluF has a distinct domain architecture, with the catalytic domain flanked at the N and C termini by additional domains connected to it by flexible linkers. The structure of the catalytic domain of RluF is similar to those of RsuA and TruB. Structural comparison of RluF with its closest structural homologues, RsuA and TruB, suggests possible functional roles for the N-terminal and C-terminal domains of RluF	Escherichia coli

Crystallization (Comment)

Organism

hanging-drop vapor-diffusion method, domain architecture and crystal structure of the catalytic domain of RluF at 2.6 A resolution. Limited proteolysis, mass spectrometry and N-terminal sequencing indicate that RluF has a distinct domain architecture, with the catalytic domain flanked at the N and C termini by additional domains connected to it by flexible linkers. The structure of the catalytic domain of RluF is similar to those of RsuA and TruB. Structural comparison of RluF with its closest structural homologues, RsuA and TruB, suggests possible functional roles for the N-terminal and C-terminal domains of RluF

Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P32684	-	-

Purification (Commentary)

Purification (Comment)	Organism
full-length RluF and N-terminal, 65-residue-truncated RulF (DELTARulF)	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
23S rRNA uridine2604	-	Escherichia coli	23S rRNA pseudouridine2604	-	?

Synonyms

Synonyms	Comment	Organism
RluF	-	Escherichia coli

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Release 2023.1 (January 2023)