BRENDA - Enzyme Database show
show all sequences of 5.4.99.2

Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein

Takahashi-Iniguez, T.; Garcia-Arellano, H.; Trujillo-Roldan, M.A.; Flores, M.E.; Biochem. Biophys. Res. Commun. 404, 443-447 (2011)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
methylmalonic acidemia protein
after 60 min of reaction, when MCM is inactive, the addition of methylmalonic acidemia protein increases the enzymatic activity through GTP hydrolysis, indicating reactivation of MCM by exchange of the damaged cofactor. Methylmalonic acidemia protein acts as a chaperone of human MCM
Homo sapiens
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
x * 80000, SDS-PAGE
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
IMAC column chromatography
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-2-methylmalonyl-CoA
-
714049
Homo sapiens
succinyl-CoA
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 80000, SDS-PAGE
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
adenosylcobalamin
MCM activity with adenosylcobalamin increases linearly during the first 3-4 min
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
methylmalonic acidemia protein
after 60 min of reaction, when MCM is inactive, the addition of methylmalonic acidemia protein increases the enzymatic activity through GTP hydrolysis, indicating reactivation of MCM by exchange of the damaged cofactor. Methylmalonic acidemia protein acts as a chaperone of human MCM
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
adenosylcobalamin
MCM activity with adenosylcobalamin increases linearly during the first 3-4 min
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
x * 80000, SDS-PAGE
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
IMAC column chromatography
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-2-methylmalonyl-CoA
-
714049
Homo sapiens
succinyl-CoA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 80000, SDS-PAGE
Homo sapiens
Other publictions for EC 5.4.99.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727036
Makins
Mutagenesis of a conserved glu ...
Homo sapiens
Biochemistry
52
878-888
2013
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727246
Ouattara
Optimization and validation of ...
Bos taurus
BMC Biochem.
14
25
2013
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726729
Han
Epimerase (Msed_0639) and muta ...
Metallosphaera sedula, Metallosphaera sedula DSM 5348
Appl. Environ. Microbiol.
78
6194-6202
2012
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726874
Buck
Treatment of a methylmalonyl-C ...
Homo sapiens
Biochem. Biophys. Res. Commun.
427
753-757
2012
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1
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728310
Chandler
Pre-clinical efficacy and dosi ...
Homo sapiens
Mol. Genet. Metab.
107
617-619
2012
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714049
Takahashi-Iniguez
Protection and reactivation of ...
Homo sapiens
Biochem. Biophys. Res. Commun.
404
443-447
2011
1
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716097
de la Fuente
Host expression of methylmalon ...
Sus scrofa
Med. Hypotheses
76
361-364
2011
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701956
Miyamoto
Characterization of methylmalo ...
Euglena gracilis, Euglena gracilis Z
Arch. Microbiol.
192
437-446
2010
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702794
Zhang
Investigating the role of nati ...
Escherichia coli
Biotechnol. Bioeng.
105
567-573
2010
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715560
Froese
Structures of the human GTPase ...
Homo sapiens
J. Biol. Chem.
285
38204-38213
2010
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691535
Nakao
Cobalamin deficiency results i ...
Rattus norvegicus
Br. J. Nutr.
101
492-498
2009
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694024
Froese
Sleeping beauty mutase (sbm) i ...
Escherichia coli
Microbiol. Res.
164
1-8
2009
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704291
Botella
Formation and metabolism of me ...
Corynebacterium glutamicum
J. Bacteriol.
191
2899-2901
2009
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704957
Mo
Enhanced FK506 production in S ...
Streptomyces clavuligerus, Streptomyces clavuligerus CKD1119
J. Ind. Microbiol. Biotechnol.
36
1473-1482
2009
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705936
Ballhausen
Evidence for catabolic pathway ...
Rattus norvegicus
Neuroscience
164
578-587
2009
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690428
Naranjo
Influence of methylmalonyl-CoA ...
Sus scrofa
Anim. Genet.
39
316-320
2008
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691201
Paizs
The putative coenzyme B12-depe ...
More
Bioorg. Chem.
36
261-264
2008
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692859
Savvi
Functional characterization of ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Bacteriol.
190
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2008
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150
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694308
Bandarian
Delivery of tailor-made cobala ...
Methylobacterium extorquens
Nat. Chem. Biol.
4
158-159
2008
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679484
Carlucci
CE assay of methylmalonyl-coen ...
Homo sapiens, Rattus norvegicus
Electrophoresis
28
1921-1925
2007
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4
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682544
Dybala-Defratyka
Coupling of hydrogenic tunneli ...
Propionibacterium freudenreichii subsp. shermanii
Proc. Natl. Acad. Sci. USA
104
10774-10779
2007
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676032
Chandler
Propionyl-CoA and adenosylcoba ...
Caenorhabditis elegans
Mol. Genet. Metab.
89
64-73
2006
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1
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677443
Deodato
Methylmalonic and propionic ac ...
Homo sapiens
Am. J. Med. Genet. C Semin. Med. Genet.
142C
104-112
2006
1
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678179
Padovani
Alternative pathways for radic ...
Propionibacterium freudenreichii subsp. shermanii
Biochemistry
45
2951-2959
2006
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2
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2
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678210
Padovani
Assembly and protection of the ...
Methylobacterium extorquens
Biochemistry
45
9300-9306
2006
-
-
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2
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2
1
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3
-
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680348
Kwiecien
Computational insights into th ...
Propionibacterium freudenreichii subsp. shermanii
J. Am. Chem. Soc.
128
1287-1292
2006
-
-
-
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-
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680659
Padovani
Energetics of interaction betw ...
Methylobacterium extorquens
J. Biol. Chem.
281
17838-17844
2006
-
-
-
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1
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662325
Kambo
Nitric oxide inhibits mammalia ...
Cricetulus sp.
J. Biol. Chem.
280
10073-10082
2005
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-
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2
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2
-
1
-
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-
-
652521
Korotkova
MeaB is a component of the met ...
Bacillus halodurans, Cupriavidus metallidurans, Geobacter metallireducens, Leptospira interrogans serovar lai str. 56601, Methylobacterium extorquens, Paraburkholderia fungorum, Propionibacterium freudenreichii subsp. shermanii, Ralstonia solanacearum, Thermobifida fusca
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18
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2
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1
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11
1
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9
-
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2
9
-
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2
10
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2
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1
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11
1
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-
-
661149
Vlasie
When a spectator turns killer: ...
Propionibacterium freudenreichii subsp. shermanii
Biochemistry
43
8410-8417
2004
-
-
1
-
3
-
2
3
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1
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1
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1
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649513
Miyamoto
Purification and characterizat ...
Sinorhizobium meliloti
Arch. Microbiol.
180
151-154
2003
-
-
-
-
-
-
4
4
-
2
2
1
-
2
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1
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1
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2
1
1
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1
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1
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1
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4
-
4
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2
2
1
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1
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-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
652252
Vlasie
Importance of the histidine li ...
Propionibacterium freudenreichii subsp. shermanii
J. Biol. Chem.
277
18523-18527
2002
-
-
1
-
2
-
-
6
-
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1
-
1
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1
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1
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3
-
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1
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1
1
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2
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6
-
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1
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1
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1
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-
3
-
-
-
-
-
-
-
-
-
-
652093
Chowdhury
The coenzyme b12 analog 5'-deo ...
Propionibacterium freudenreichii subsp. shermanii
J. Biol. Chem.
276
1015-1019
2001
-
-
1
-
-
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1
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1
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1
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2
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1
2
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1
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1
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1
-
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-
2
-
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-
-
-
-
-
-
-
-
649999
Chowdhury
Thermodynamic and kinetic char ...
Propionibacterium freudenreichii subsp. shermanii
Biochemistry
39
7998-8006
2000
-
-
1
-
-
-
-
-
-
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-
1
-
1
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1
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1
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1
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1
1
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650005
Thoma
Protection of radical intermed ...
Propionibacterium freudenreichii subsp. shermanii
Biochemistry
39
9213-9221
2000
-
-
1
1
2
-
-
3
-
-
-
1
-
1
-
-
1
-
-
-
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1
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4
-
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1
-
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1
1
1
2
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3
-
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1
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1
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
649878
Chowdhury
Role of the dimethylbenzimidaz ...
Propionibacterium freudenreichii subsp. shermanii
Biochemistry
38
15287-15294
1999
-
-
1
-
-
-
-
-
-
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1
-
1
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1
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-
1
-
1
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1
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1
1
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1
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1
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-
1
-
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
649904
Mancia
Crystal structure of substrate ...
Propionibacterium freudenreichii subsp. shermanii
Biochemistry
38
7999-8005
1999
-
-
1
1
-
-
2
-
-
-
-
1
-
1
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-
1
-
-
-
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1
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1
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1
1
1
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2
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1
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-
1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3514
Mancia F.; Evans
Conformational changes on subs ...
Propionibacterium freudenreichii subsp. shermanii
Structure
6
711-720
1998
-
-
1
1
-
-
-
-
-
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-
1
-
2
-
-
1
-
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-
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1
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1
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1
1
1
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1
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1
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3515
Poppe
A base-off analogue of coenzym ...
Propionibacterium freudenreichii subsp. shermanii
Eur. J. Biochem.
250
303-307
1997
1
-
-
-
-
-
-
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1
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1
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1
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1
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1
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-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3516
Abend
Further insights into the mech ...
Propionibacterium freudenreichii subsp. shermanii
Eur. J. Biochem.
249
180-186
1997
-
-
-
-
-
-
5
1
-
-
-
-
-
1
-
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
1
-
-
-
-
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-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3518
Janata
Expression and kinetic charact ...
Homo sapiens
Hum. Mol. Genet.
6
1457-1464
1997
-
-
1
-
6
-
-
-
1
-
-
2
-
3
-
-
-
-
-
-
-
-
3
-
-
-
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1
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1
1
-
6
-
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1
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3511
Mancia
How coenzyme B12 radicals are ...
Propionibacterium freudenreichii subsp. shermanii
Structure
4
339-350
1996
-
-
-
1
-
-
-
-
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-
-
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2
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1
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1
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3513
Taoka
Inhibition of the human methyl ...
Homo sapiens
J. Biol. Chem.
269
31630-31634
1994
-
-
-
-
-
-
3
1
-
-
-
-
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2
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-
1
-
-
-
1
-
2
-
-
-
-
2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3517
Zhao
Electron paramagnetic resonanc ...
Propionibacterium freudenreichii subsp. shermanii
Eur. J. Biochem.
225
891-896
1994
-
-
-
-
-
-
1
4
-
-
-
-
-
1
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3494
Wilkemeyer
Primary structure and activity ...
Mus musculus
Biochem. J.
271
449-455
1990
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3510
McKie
Adenosylcobalamin-dependent me ...
Propionibacterium freudenreichii subsp. shermanii
Biochem. J.
269
293-298
1990
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3512
Marsh
Cloning and structural charact ...
Propionibacterium freudenreichii subsp. shermanii
Biochem. J.
260
345-352
1989
-
-
1
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3507
Marsh
Crystallization and preliminar ...
Propionibacterium freudenreichii subsp. shermanii
J. Mol. Biol.
200
421-422
1988
-
-
-
1
-
-
-
-
-
-
-
-
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2
-
-
-
-
-
-
-
-
1
-
-
-
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-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3508
Ledley
Molecular cloning of L-methylm ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
85
3518-3521
1988
-
-
1
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3509
Kolhouse
L-Methylmalonyl-CoA mutase fro ...
Homo sapiens
Methods Enzymol.
166
407-414
1988
-
-
-
-
-
-
-
1
-
-
2
-
-
2
-
-
1
-
-
2
1
-
1
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
2
-
-
-
-
1
-
2
1
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3505
Michenfelder
-
Methylmalonyl-carba(dethia)-co ...
Mammalia
Angew. Chem.
98
337-338
1986
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
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-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3506
Francalanci
The subunit structure of methy ...
Propionibacterium freudenreichii subsp. shermanii
Biochem. J.
236
489-494
1986
-
-
-
-
-
-
-
-
-
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3
-
-
2
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-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3504
Hunaiti
Source of methylmalonyl-coenzy ...
Saccharopolyspora erythraea
Antimicrob. Agents Chemother.
25
173-178
1984
-
-
-
-
-
-
-
1
-
-
1
1
-
2
-
-
1
-
-
-
1
1
3
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
-
1
1
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3502
Retey
-
Methylmalonyl-CoA mutase ...
Mammalia, Ovis aries, Propionibacterium freudenreichii subsp. shermanii
B12 (Dolphin, D. ed. ) Wiley, New York
2
357-379
1982
-
-
-
-
-
-
2
4
-
-
4
2
-
3
-
-
2
2
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1
2
1
6
1
-
-
-
-
2
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7
-
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7
-
-
-
-
2
-
4
-
-
4
2
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-
-
2
-
1
2
1
6
1
-
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2
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3503
Fenton
Purification and properties of ...
Homo sapiens
Arch. Biochem. Biophys.
214
815-823
1982
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1
3
1
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3
2
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2
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1
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2
1
1
2
1
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2
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1
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1
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1
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1
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3
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1
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3
2
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1
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2
1
1
2
1
-
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2
-
1
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3501
Gaudemer
Reversible cleavage of the cob ...
Propionibacterium freudenreichii subsp. shermanii
Eur. J. Biochem.
119
279-285
1981
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-
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2
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1
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1
-
-
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3500
Kolhouse
Isolation and characterization ...
Homo sapiens
J. Biol. Chem.
255
2708-2712
1980
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-
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2
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3
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2
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1
1
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1
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1
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2
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2
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1
1
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3409
Poston
-
Coenzyme B12-dependent enzymes ...
Solanum tuberosum
Phytochemistry
17
401-402
1978
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-
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1
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1
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1
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1
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1
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1
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1
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1
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1
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3519
Retey
Investigation of the mechanism ...
Propionibacterium freudenreichii subsp. shermanii
Eur. J. Biochem.
83
437-451
1978
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2
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1
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1
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1
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1
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1
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3497
Kolhouse
Recognition of two intracellul ...
Oryctolagus cuniculus
Proc. Natl. Acad. Sci. USA
74
921-925
1977
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1
1
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3
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1
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1
1
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1
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2
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3498
Murthy
Purification of methylmalonyl- ...
Propionibacterium freudenreichii subsp. shermanii
Biochim. Biophys. Acta
483
487-491
1977
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-
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2
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1
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1
1
1
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1
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1
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1
1
1
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1
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3496
Sato
Methylmalonyl-CoA mutase in a ...
Serratia plymuthica
FEBS Lett.
71
248-250
1976
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2
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4
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1
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1
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1
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4
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1
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3495
Zagalak
Studies on methylmalonyl-CoA m ...
Propionibacterium freudenreichii subsp. shermanii
Eur. J. Biochem.
44
529-535
1974
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1
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3
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2
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1
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1
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1
1
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1
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3
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1
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1
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1
1
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