Literature summary for 5.4.99.2 extracted from

Kambo, A.; Sharma, V.S.; Casteel, D.E.; Woods, V.L., Jr.; Pilz, R.B.; Boss, G.R.
Nitric oxide inhibits mammalian methylmalonyl-CoA mutase (2005), J. Biol. Chem., 280, 10073-10082.

Search for more literature for 5.4.99.2

Inhibitors

Inhibitors	Comment	Organism	Structure
2-(N,N-diethylamino)-diazenolate-2-oxide	1 mM, about 50% inhibition of enzyme activity. Similar inhibitory effects of 2-(N,N-diethylamino)-diazenolate-2-oxide in the presence of 25 mM glutathione and dithiothreitol	Cricetulus sp.
oxygen	600 microM, significant inhibition of enzyme activity	Cricetulus sp.

Organism

Organism	UniProt	Comment	Textmining
Cricetulus sp.	-	Baby hamster kidney fibroblast like cells	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Cricetulus sp.	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.000052	-	baby hamster kidney cells grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, lacking hydroxycobalamin, 37°C, pH 7.4	Cricetulus sp.
0.0002	-	cell extracts preincubated with 5 microM adenosylcobalamin, 37°C, pH 7.4	Cricetulus sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methylmalonyl-CoA	-	Cricetulus sp.	succinyl-CoA	-	r

Cofactor

Cofactor	Comment	Organism	Structure
adenosylcobalamin	-	Cricetulus sp.

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Release 2023.1 (January 2023)