Literature summary for 5.4.99.2 extracted from

Thoma, N.H.; Evans, P.R.; Leadlay, P.F.
Protection of radical intermediates at the active site of adenosylcobalamin-dependent methylmalonyl-CoA mutase (2000), Biochemistry, 39, 9213-9221.

Search for more literature for 5.4.99.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Propionibacterium freudenreichii subsp. shermanii

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop method	Propionibacterium freudenreichii subsp. shermanii

Protein Variants

Protein Variants	Comment	Organism
H224Q	lower turnover than wild-type enzyme	Propionibacterium freudenreichii subsp. shermanii
H244A	lower turnover than wild-type enzyme	Propionibacterium freudenreichii subsp. shermanii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.083	-	succinyl-CoA	25°C, mutant H244A	Propionibacterium freudenreichii subsp. shermanii
0.085	-	succinyl-CoA	25°C, mutant H244Q	Propionibacterium freudenreichii subsp. shermanii
0.096	-	succinyl-CoA	25°C, wild-type	Propionibacterium freudenreichii subsp. shermanii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(R)-2-methylmalonyl-CoA	Propionibacterium freudenreichii subsp. shermanii	-	succinyl-CoA	-	r

Organism

Organism	UniProt	Comment	Textmining
Propionibacterium freudenreichii subsp. shermanii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Propionibacterium freudenreichii subsp. shermanii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-2-methylmalonyl-CoA	-	Propionibacterium freudenreichii subsp. shermanii	succinyl-CoA	-	r

Synonyms

Synonyms	Comment	Organism
methylmalonyl-CoA mutase	-	Propionibacterium freudenreichii subsp. shermanii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.038	-	succinyl-CoA	25°C, mutant H244A	Propionibacterium freudenreichii subsp. shermanii
0.66	-	succinyl-CoA	25°C, mutant H244Q	Propionibacterium freudenreichii subsp. shermanii
6.08	-	succinyl-CoA	25°C, mutant H244Q	Propionibacterium freudenreichii subsp. shermanii
48	-	succinyl-CoA	25°C, wild-type	Propionibacterium freudenreichii subsp. shermanii

Cofactor

Cofactor	Comment	Organism	Structure
adenosylcobalamin	-	Propionibacterium freudenreichii subsp. shermanii

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Release 2023.1 (January 2023)