Literature summary for 5.4.99.19 extracted from

Sivaraman, J.; Sauve, V.; Larocque, R.; Stura, E.A.; Schrag, J.D.; Cygler, M.; Matte, A.
Structure of the 16S rRNA pseudouridine synthase RsuA bound to uracil and UMP (2002), Nat. Struct. Biol., 9, 353-358.

Search for more literature for 5.4.99.19

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion at 18°C, crystal structure of RsuA bound to uracil at 2.0 A resolution and to uridine 5'-monophosphate at 2.65 A resolution. RsuA consists of an N-terminal domain connected by an extended linker to the central and C-terminal domains. Uracil and UMP bind in a cleft between the central and C-terminal domains near the catalytic residue Asp102. The N-terminal domain shows structural similarity to the ribosomal protein S4. Despite only 15% amino acid identity, the other two domains are structurally similar to those of the tRNA-specific Psi-synthase TruA, including the position of the catalytic Asp	Escherichia coli

Crystallization (Comment)

Organism

sitting drop vapor diffusion at 18°C, crystal structure of RsuA bound to uracil at 2.0 A resolution and to uridine 5'-monophosphate at 2.65 A resolution. RsuA consists of an N-terminal domain connected by an extended linker to the central and C-terminal domains. Uracil and UMP bind in a cleft between the central and C-terminal domains near the catalytic residue Asp102. The N-terminal domain shows structural similarity to the ribosomal protein S4. Despite only 15% amino acid identity, the other two domains are structurally similar to those of the tRNA-specific Psi-synthase TruA, including the position of the catalytic Asp

Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0AA43	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Synonyms

Synonyms	Comment	Organism
PSI-synthase RsuA	-	Escherichia coli
RsuA	-	Escherichia coli

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Release 2023.1 (January 2023)