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Literature summary for 5.4.99.17 extracted from
- Selectivity in the cyclization of citronellal introduced by squalene hopene cyclase variants (2017), ChemCatChem, 9, 4364-4368 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Alicyclobacillus acidocaldarius |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D376C | inactive | Alicyclobacillus acidocaldarius |
| I261A | the mutant has higher activity with (S)-citronellal compared to the wild type enzyme | Alicyclobacillus acidocaldarius |
| W169A | the mutant has higher activity with (S)-citronellal compared to the wild type enzyme | Alicyclobacillus acidocaldarius |
| W312G | the mutant produces (-)-neo-isopulegol from (S)-citronellal | Alicyclobacillus acidocaldarius |
| Y609A | the mutant produces (+)-isopulegol from (S)-citronellal | Alicyclobacillus acidocaldarius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| squalene | Alicyclobacillus acidocaldarius | - |
hop-22(29)-ene | - |
? |  |
| squalene + H2O | Alicyclobacillus acidocaldarius | - |
hopan-22-ol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alicyclobacillus acidocaldarius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sephacel column chromatography | Alicyclobacillus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-citronellal + H2O | - |
Alicyclobacillus acidocaldarius | (-)-isopulegol + (+)-neoiso-isopulegol | - |
? | |
| (S)-citronellal + H2O | - |
Alicyclobacillus acidocaldarius | (+)-isopulegol | - |
? | |
| squalene | - |
Alicyclobacillus acidocaldarius | hop-22(29)-ene | - |
? | |
| squalene + H2O | - |
Alicyclobacillus acidocaldarius | hopan-22-ol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SHC | - |
Alicyclobacillus acidocaldarius |
| squalene hopene cyclase | - |
Alicyclobacillus acidocaldarius |
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Release 2023.1 (January 2023)
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