Protein Variants | Comment | Organism |
---|---|---|
F365W | marginal catalytic activity | Alicyclobacillus acidocaldarius |
F365Y | and mutant with F365 changed to unnatural amino acid O-methyltyrosine. Both show increased decreased activity at high temperature | Alicyclobacillus acidocaldarius |
F605W | increased catalytic acitivy at low temperature, but decreased activity at high temperature due to higher cation-pi binding energies | Alicyclobacillus acidocaldarius |
F605Y | and mutant with F605 changed to unnatural amino acid O-methyltyrosine. Both show increased catalytic acitivy at low temperature, but decreased activity at high temperature due to higher cation-pi binding energies | Alicyclobacillus acidocaldarius |
additional information | replacement of F605 by mono-, di- or trifluorophenylalanine, with or without additional mutation Y606A, kinetic analysis. Mutant F605 changed to trifluorophenylalanine plus mutation Y606A has negligibly small activity | Alicyclobacillus acidocaldarius |
Y606A | kinetics identical to wild-type | Alicyclobacillus acidocaldarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0154 | - |
squalene | wild-type, pH 6.0, 40°C | Alicyclobacillus acidocaldarius | |
0.0242 | - |
squalene | wild-type, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.0279 | - |
squalene | mutant F605 changed to fluorophenylalanine plus mutation Y606A, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.0324 | - |
squalene | mutant F605 changed to difluorophenylalanine plus mutation Y606A, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.0352 | - |
squalene | mutant F365 changed to fluorophenylalanine, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.0416 | - |
squalene | mutant F365 changed to difluorophenylalanine, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.0479 | - |
squalene | mutant F6365 changed to trifluorophenylalanine, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.0525 | - |
squalene | mutant F605Y, pH 6.0, 40°C | Alicyclobacillus acidocaldarius | |
0.104 | - |
squalene | mutant F605Y, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.105 | - |
squalene | mutant F605 changed to O-methyltyrosine, pH 6.0, 40°C | Alicyclobacillus acidocaldarius | |
0.118 | - |
squalene | mutant F605W, pH 6.0, 40°C | Alicyclobacillus acidocaldarius | |
0.13 | - |
squalene | mutant F365Y, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.152 | - |
squalene | mutant F605Y changed to O-methyltyrosine, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.185 | - |
squalene | mutant F365Y changed to O-methyltyrosine, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.191 | - |
squalene | mutant F605W, pH 6.0, 50°C | Alicyclobacillus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alicyclobacillus acidocaldarius | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
squalene | - |
Alicyclobacillus acidocaldarius | hop-22(29)-ene | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
wild-type | Alicyclobacillus acidocaldarius |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
squalene | mutant F365Y changed to O-methyltyrosine, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.17 | - |
squalene | mutant F365Y, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.25 | - |
squalene | wild-type, pH 6.0, 40°C | Alicyclobacillus acidocaldarius | |
0.27 | - |
squalene | mutant F605Y, pH 6.0, 40°C | Alicyclobacillus acidocaldarius | |
0.31 | - |
squalene | mutant F605W, pH 6.0, 40°C | Alicyclobacillus acidocaldarius | |
0.45 | - |
squalene | mutant F605 changed to O-methyltyrosine, pH 6.0, 40°C | Alicyclobacillus acidocaldarius | |
0.54 | - |
squalene | mutant F605 changed to difluorophenylalanine plus mutation Y606A, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.7 | - |
squalene | mutant F605Y changed to O-methyltyrosine, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.75 | - |
squalene | mutant F6365 changed to trifluorophenylalanine, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.82 | - |
squalene | mutant F605 changed to fluorophenylalanine plus mutation Y606A, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
0.92 | - |
squalene | mutant F365 changed to difluorophenylalanine, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
1.1 | - |
squalene | mutant F365 changed to fluorophenylalanine, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
1.32 | - |
squalene | mutant F605W, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
1.82 | - |
squalene | mutant F605Y, pH 6.0, 50°C | Alicyclobacillus acidocaldarius | |
1.98 | - |
squalene | wild-type, pH 6.0, 50°C | Alicyclobacillus acidocaldarius |