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Literature summary for 5.4.99.16 extracted from
- Inducible expression of trehalose synthase in Bacillus licheniformis (2017), Protein Expr. Purif., 130, 115-122 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| plasmid-based inducible expression systems are constructed to produce the Thermomonospora curvata enzyme in Bacillus licheniformis. Xylose operons rom Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium are introduced to regulate the expression of the gene encoding TreS. It is functionally expressed, and the construct yields enzyme activity of 12.1 U/mL | Thermomonospora curvata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermomonospora curvata | D1ABU6 | - |
- |
| Thermomonospora curvata ATCC 19995 | D1ABU6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermomonospora curvata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose | - |
Thermomonospora curvata | maltose | - |
r |  |
| alpha,alpha-trehalose | - |
Thermomonospora curvata ATCC 19995 | maltose | - |
r | |
| maltose | - |
Thermomonospora curvata | alpha,alpha-trehalose | - |
r | |
| maltose | - |
Thermomonospora curvata ATCC 19995 | alpha,alpha-trehalose | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Trehalose synthase | - |
Thermomonospora curvata |
| TreS | - |
Thermomonospora curvata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Thermomonospora curvata |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 50 | 15°C: about 45% of maximal activity, 50°C: about 85% of maximal activity | Thermomonospora curvata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Thermomonospora curvata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 7.8 | pH 5.8: about 50% of maximal activity, pH 7.8: about 50% of maximal activity | Thermomonospora curvata |
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Release 2023.1 (January 2023)
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