Literature summary for 5.4.99.16 extracted from

Sun, J.; Wang, S.; Li, W.; Li, R.; Chen, S.; Ri, H.; Kim, T.; Kang, M.; Sun, L.; Sun, X.; Yuan, Q.
Improvement of trehalose production by immobilized trehalose synthase from Thermus thermophilus HB27 (2018), Molecules, 23, pii: E1087 .

Search for more literature for 5.4.99.16

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant enzyme expression in Escherichia coli strain BL21(DE3)	Thermus thermophilus

Protein Variants

Protein Variants	Comment	Organism
additional information	the crude TtTreS is immobilized on the surface of glutaraldehyde-3-aminopropyltriethoxysilane-silicalite-1 (GA-APS-silicalite-1, silicalite-1 modified sequentially with 3-aminopropyltriethoxysilane and glutaraldehyde) without enzyme purification. In this case, a nucleophilic attack takes place at the aldehyde group of glutaraldehyde by the amino group of the protein to form a Schiff base. Preparation of activated silicalite-1, APS-silicalite-1, and GA-APS-silicalite-1 and enzyme immobilization, method optimization, overview. Immobilization also provides TtTreS with potential application in a relatively wide range of temperatures from 40°C to 70°C. The trehalose yield of immobilized TtTreS in first cycle reaches 61.52%. After 22 cycles of enzymatic reaction, the immobilized TtTreS still retains 81% of its initial trehalose yield	Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
14.12	-	maltose	recombinant free enzyme, pH 8.0, 50°C	Thermus thermophilus
53.33	-	maltose	recombinant immobilized enzyme, pH 8.0, 50°C	Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
maltose	Thermus thermophilus	-	alpha,alpha-trehalose	-	r

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	O06458	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
maltose	-	Thermus thermophilus	alpha,alpha-trehalose	-	r

Synonyms

Synonyms	Comment	Organism
Trehalose synthase	-	Thermus thermophilus
TtTreS	-	Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	free and immobilized recombinant enzyme	Thermus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	immobilized recombinant enzyme	Thermus thermophilus
9	-	free recombinant enzyme	Thermus thermophilus

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Release 2023.1 (January 2023)