Literature summary for 5.4.99.16 extracted from

Li, Y.; Sun, X.; Feng, Y.; Yuan, Q.
Cloning, expression and activity optimization of trehalose synthase from Thermus thermophilus HB27 (2017), Chem. Eng. Sci., 135, 323-329 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
Ca2+	recombinant enzyme by 19%	Thermus thermophilus
DTT	recombinant enzyme by 41%	Thermus thermophilus

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3). Plasmids with different promoters and copy numbers are important for the expression of trehalose synthase genes in Escherichia coli. The trehalose synthase from Thermus thermophilus is best expressed from plasmid pET-22b showing high activity in trehalose production with 1.996 U/mg protein, co-expression of molecular chaperones sigma32, GroEL, GroES, DnaK and DnaJ greatly increasing the solubility of the trehalose synthase protein	Thermus thermophilus

Cloned (Comment)

Organism

recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3). Plasmids with different promoters and copy numbers are important for the expression of trehalose synthase genes in Escherichia coli. The trehalose synthase from Thermus thermophilus is best expressed from plasmid pET-22b showing high activity in trehalose production with 1.996 U/mg protein, co-expression of molecular chaperones sigma32, GroEL, GroES, DnaK and DnaJ greatly increasing the solubility of the trehalose synthase protein

Thermus thermophilus

Protein Variants

Protein Variants	Comment	Organism
additional information	enzyme expression in Escherichia coli strain Rosetta (DE3) for trehalose production, method optimization to 50°C, pH 9.0, 10 h, 10% maltose solution, and co-expression of molecular chaperones sigma32, GroEL, GroES, DnaK and DnaJ greatly increasing the solubility of the trehalose synthase protein. Addition of Ca2+ and DTT increase the activity by 19% and 41%, respectively	Thermus thermophilus

Inhibitors

Inhibitors	Comment	Organism
Mg2+	about 50% inhibition	Thermus thermophilus
Mn2+	85% inhibition	Thermus thermophilus
Zn2+	80% inhibition	Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
maltose	Thermus thermophilus	-	alpha,alpha-trehalose	-	r

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
maltose	-	Thermus thermophilus	alpha,alpha-trehalose	-	r

Synonyms

Synonyms	Comment	Organism
Trehalose synthase	-	Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	recombinant enzyme	Thermus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	recombinant enzyme	Thermus thermophilus