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Literature summary for 5.4.99.16 extracted from
- Homology modeling and function of trehalose synthase from Pseudomonas putida P06 (2014), Biotechnol. Lett., 36, 1009-1013.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene treS, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D112A | site-directed mutagenesis, inactive mutant | Pseudomonas putida |
| D294A | site-directed mutagenesis, inactive mutant | Pseudomonas putida |
| D403A | site-directed mutagenesis, inactive mutant | Pseudomonas putida |
| E338A | site-directed mutagenesis, inactive mutant | Pseudomonas putida |
| F115A | site-directed mutagenesis, inactive mutant | Pseudomonas putida |
| F223A | site-directed mutagenesis, the mutation only modestly affects the enzymatic activity | Pseudomonas putida |
| F255A | site-directed mutagenesis, inactive mutant | Pseudomonas putida |
| Q259A | site-directed mutagenesis, the mutant shows 70% reduced activity compared to the wild-type enzyme | Pseudomonas putida |
| R535A | site-directed mutagenesis, the mutation only modestly affects the enzymatic activity | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
x * 75000 | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | Pseudomonas putida | - |
alpha,alpha-trehalose | - |
r |  |
| maltose | Pseudomonas putida P06 | - |
alpha,alpha-trehalose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
gene treS | - |
| Pseudomonas putida P06 | - |
gene treS | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | - |
Pseudomonas putida | alpha,alpha-trehalose | - |
r | |
| maltose | - |
Pseudomonas putida P06 | alpha,alpha-trehalose | - |
r | |
| additional information | TreS interconverts maltose and trehalose by an intramolecular mechanism, and therefore does not require external glucose or any other factors during the enzymatic reaction | Pseudomonas putida | ? | - |
? | |
| additional information | TreS interconverts maltose and trehalose by an intramolecular mechanism, and therefore does not require external glucose or any other factors during the enzymatic reaction | Pseudomonas putida P06 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 75000 | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Trehalose synthase | - |
Pseudomonas putida |
| TreS | - |
Pseudomonas putida |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the highly conserved Asp294 residue is critical for catalytic activity, homology modeling and flexible docking studies of the enzyme-substrate system, overview | Pseudomonas putida |
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Release 2023.1 (January 2023)
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