Cloned (Comment) | Organism |
---|---|
locus DR-2036, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of C-terminally His6-tagged enzyme in Escherichia coli strain (DE3) Rosetta pLysS from pET30Ek/LIC vector | Deinococcus radiodurans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 98.5% inhibition at 1 mM | Deinococcus radiodurans | |
Ca2+ | 13.4% inhibition at 10 mM, 9.5% at 1 mM | Deinococcus radiodurans | |
Co2+ | 17.2% inhibition at 1 mM | Deinococcus radiodurans | |
Cu2+ | complete inhibition at 10 mM | Deinococcus radiodurans | |
dithiothreitol | 9% inhibition at 5 mM, 12% at 10 mM | Deinococcus radiodurans | |
EDTA | 15% inhibition at 1 mM, 35% at 10 mM | Deinococcus radiodurans | |
Hg2+ | complete inhibition at 1 mM | Deinococcus radiodurans | |
Mg2+ | 8.5% inhibition at 1-10 mM | Deinococcus radiodurans | |
Mn2+ | 20% inhibition at 1 mM, 30% at 10 mM | Deinococcus radiodurans | |
Ni2+ | 28.5% inhibition at 1mM, 45% at 10 mM | Deinococcus radiodurans | |
SDS | 95.1% inhibition at 5 mM | Deinococcus radiodurans | |
Tris | 58% inhibition at 5 mM, 78% at 10 mM | Deinococcus radiodurans | |
Zn2+ | complete inhibition at 5 mM | Deinococcus radiodurans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
290.7 | - |
maltose | pH 7.6, 30°C, recombinant His6-tagged enzyme | Deinococcus radiodurans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62710 | - |
2 * 62710, sequence calculation, 2 * 64690, recombinant His6-tagged enzyme, sequence calculation, 2 * 64000, recombinant His6-tagged enzyme, SDS-PAGE | Deinococcus radiodurans |
64000 | - |
2 * 62710, sequence calculation, 2 * 64690, recombinant His6-tagged enzyme, sequence calculation, 2 * 64000, recombinant His6-tagged enzyme, SDS-PAGE | Deinococcus radiodurans |
64690 | - |
2 * 62710, sequence calculation, 2 * 64690, recombinant His6-tagged enzyme, sequence calculation, 2 * 64000, recombinant His6-tagged enzyme, SDS-PAGE | Deinococcus radiodurans |
126900 | - |
recombinant His6-tagged enzyme, gel filtration | Deinococcus radiodurans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose | Deinococcus radiodurans | - |
alpha,alpha-trehalose | - |
? | |
maltose | Deinococcus radiodurans DSM Z 20539 | - |
alpha,alpha-trehalose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus radiodurans | Q9RST7 | locus DR-2036 | - |
Deinococcus radiodurans DSM Z 20539 | Q9RST7 | locus DR-2036 | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain (DE3) Rosetta pLysS by cobalt affinity chromatography and ultrafiltration | Deinococcus radiodurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose | - |
Deinococcus radiodurans | alpha,alpha-trehalose | - |
? | |
maltose | - |
Deinococcus radiodurans DSM Z 20539 | alpha,alpha-trehalose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 62710, sequence calculation, 2 * 64690, recombinant His6-tagged enzyme, sequence calculation, 2 * 64000, recombinant His6-tagged enzyme, SDS-PAGE | Deinococcus radiodurans |
Synonyms | Comment | Organism |
---|---|---|
Trehalose synthase | - |
Deinococcus radiodurans |
TreS | - |
Deinococcus radiodurans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Deinococcus radiodurans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 50 | activity range, profile, overview | Deinococcus radiodurans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
purified recombinant His6-tagged enzyme, 2 h, 45°C and pH 7.6, stable up to | Deinococcus radiodurans |
50 | - |
purified recombinant His6-tagged enzyme, 2 h, pH 7.6, inactivation | Deinococcus radiodurans |
50 | - |
purified recombinant His6-tagged enzyme, 8 h, pH 7.6, 56% activity remaining | Deinococcus radiodurans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Deinococcus radiodurans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | activity range, profile, overview | Deinococcus radiodurans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
purified recombinant His6-tagged enzyme, 2 h, 30°C, inactivation | Deinococcus radiodurans |
5.5 | - |
purified recombinant His6-tagged enzyme, 2 h, 30°C, 50% activity remaining | Deinococcus radiodurans |
7.6 | - |
purified recombinant His6-tagged enzyme, 2 h, 45°C and pH 7.6, stable | Deinococcus radiodurans |
9.5 | - |
purified recombinant His6-tagged enzyme, 2 h, 30°C, 50% activity remaining | Deinococcus radiodurans |
10 | - |
purified recombinant His6-tagged enzyme, 2 h, 30°C, inactivation | Deinococcus radiodurans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Deinococcus radiodurans | sequence calculation | - |
4.94 |