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Literature summary for 5.4.99.15 extracted from

  • Maruta, K.; Kubota, M.; Yamashita, H.; Nishimoto, T.; Chaen, H.; Fukuda, S.
    Creation of a novel hydrolase by site-directed mutagenesis of malto-oligosyltrehalose synthase (2006), J. Appl. Glycosci., 53, 199-203.
No PubMed abstract available
Search for more literature for 5.4.99.15

Protein Variants

Protein Variants Comment Organism
D228N complete loss of both hydrolytic and transglycosylation activity Sulfolobus acidocaldarius
D231A significant decrease of both hydrolytic and transglycosylation activity Sulfolobus acidocaldarius
D275A about 50% and 40% decrease of hydrolytic and transglycosylation activity, resp. Sulfolobus acidocaldarius
D394A significant decrease of both hydrolytic and transglycosylation activity Sulfolobus acidocaldarius
D443N complete loss of both hydrolytic and transglycosylation activity Sulfolobus acidocaldarius
E255Q complete loss of both hydrolytic and transglycosylation activity Sulfolobus acidocaldarius
E393A complete loss of both hydrolytic and transglycosylation activity Sulfolobus acidocaldarius
H229N complete loss of both hydrolytic and transglycosylation activity Sulfolobus acidocaldarius
K256T significant decrease of both hydrolytic and transglycosylation activity Sulfolobus acidocaldarius
K390A increase in hydrolytic, decrease in transglycosylation activity Sulfolobus acidocaldarius
K390E increase in hydrolytic, decrease in transglycosylation activity Sulfolobus acidocaldarius
K390H increase in hydrolytic, decrease in transglycosylation activity Sulfolobus acidocaldarius
K390Q increase in hydrolytic, decrease in transglycosylation activity Sulfolobus acidocaldarius
K390R increase in hydrolytic, decrease in transglycosylation activity Sulfolobus acidocaldarius
K390T increase in hydrolytic, decrease in transglycosylation activity Sulfolobus acidocaldarius
K390T increase in hydrolytic, significant decrease in transglycosylation activity Sulfolobus acidocaldarius
K390W complete loss of transglycosylation activity, increase in hydrolytic activity Sulfolobus acidocaldarius
K390W/K445W complete loss of transglycosylation activity, 40% decrease in hydrolytic activity Sulfolobus acidocaldarius
K445A increase in hydrolytic, decrease in transglycosylation activity Sulfolobus acidocaldarius
K445E increase in hydrolytic, decrease in transglycosylation activity Sulfolobus acidocaldarius
K445H increase in hydrolytic, decrease in transglycosylation activity Sulfolobus acidocaldarius
K445Q increase in hydrolytic, decrease in transglycosylation activity Sulfolobus acidocaldarius
K445R increase in hydrolytic, decrease in transglycosylation activity Sulfolobus acidocaldarius
K445T increase in hydrolytic, significant decrease in transglycosylation activity Sulfolobus acidocaldarius
K445W complete loss of transglycosylation activity, increase in hydrolytic activity Sulfolobus acidocaldarius
R192A about 50% and 40% decrease of hydrolytic and transglycosylation activity, resp. Sulfolobus acidocaldarius
R402S about 30% decrease of both hydrolytic and transglycosylation activity Sulfolobus acidocaldarius
Y274S significant decrease of both hydrolytic and transglycosylation activity Sulfolobus acidocaldarius
Y400S about 25% and 20% decrease of hydrolytic and transglycosylation activity, resp. Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
 strain ATCC 33909
-