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Literature summary for 5.4.99.15 extracted from

  • Fang, T.Y.; Tseng, W.C.; Pan, C.H.; Chun, Y.T.; Wang, M.Y.
    Protein engineering of Sulfolobus solfataricus maltooligosyltrehalose synthase to alter its selectivity (2007), J. Agric. Food Chem., 55, 5588-5594.
    View publication on PubMed
Search for more literature for 5.4.99.15

Protein Variants

Protein Variants Comment Organism
F206W significant decrease in transglycosylation activity Saccharolobus solfataricus
F206Y significant decrease in transglycosylation activity Saccharolobus solfataricus
F207Y significant decrease in transglycosylation activity Saccharolobus solfataricus
F405M catalytic activity comparable to wild-type Saccharolobus solfataricus
F405S depending on substrate, 29-97% of wild-type transglycosylation efficiency, hydrolytic activity is 16% of wild-type Saccharolobus solfataricus
F405W catalytic activity slightly lower than wild-type Saccharolobus solfataricus
F405Y decreased ratio of hydrolysis to transglycosylation Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.93
-
 maltohexaose mutant F206W, pH 5.0, 60°C Saccharolobus solfataricus
2.48
-
 maltohexaose mutant F206Y, pH 5.0, 60°C Saccharolobus solfataricus
2.84
-
 maltoheptaose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
2.92
-
 maltohexaose mutant F207Y, pH 5.0, 60°C Saccharolobus solfataricus
3.38
-
 maltoheptaose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus
3.7 5 maltohexaose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
3.8
-
 maltoheptaose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
3.88
-
 maltoheptaose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
4.53
-
 maltohexaose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
4.63
-
 maltohexaose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus
4.9
-
 maltohexaose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
5.71
-
 maltoheptaose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus
5.9
-
 maltohexaose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus
5.94
-
 maltopentaose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
6.34
-
 maltopentaose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
6.6
-
 maltopentaose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus
7.9
-
 maltopentaose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus
9.25
-
 maltopentaose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
11.6
-
 maltotetraose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
13.2
-
 maltotetraose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
19
-
 maltotetraose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus
20.2
-
 maltotetraose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
20.6
-
 maltotetraose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose
-
 Saccharolobus solfataricus alpha-maltopentaosyl trehalose
-
 ?
maltohexaose
-
 Saccharolobus solfataricus alpha-maltotetraosyl trehalose
-
 ?
Maltopentaose
-
 Saccharolobus solfataricus alpha-Maltotriosyl trehalose
-
 ?
Maltotetraose
-
 Saccharolobus solfataricus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.11
-
 maltohexaose mutant F206Y, pH 5.0, 60°C Saccharolobus solfataricus
11.1
-
 maltohexaose mutant F206W, pH 5.0, 60°C Saccharolobus solfataricus
14
-
 maltohexaose mutant F207Y, pH 5.0, 60°C Saccharolobus solfataricus
61
-
 maltotetraose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
138
-
 maltotetraose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
139
-
 maltotetraose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus
139
-
 maltotetraose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
147
-
 maltoheptaose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
169
-
 maltohexaose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
196
-
 maltotetraose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus
197
-
 maltopentaose mutant F405S, pH 5.0, 60°C Saccharolobus solfataricus
354
-
 maltopentaose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
359
-
 maltohexaose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
362
-
 maltohexaose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
363
-
 maltoheptaose mutant F405Y, pH 5.0, 60°C Saccharolobus solfataricus
364
-
 maltopentaose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
381
-
 maltoheptaose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus
383
-
 maltoheptaose wild-type, pH 5.0, 60°C Saccharolobus solfataricus
410
-
 maltopentaose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus
414
-
 maltohexaose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus
429
-
 maltoheptaose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus
445
-
 maltohexaose mutant F405W, pH 5.0, 60°C Saccharolobus solfataricus
481
-
 maltopentaose mutant F405M, pH 5.0, 60°C Saccharolobus solfataricus