Literature summary for 5.4.99.15 extracted from

  • Nakada, T.; Maruta, K.; Tsusaki, K.; Kubota, M.; Chaen, H.; Sugimoto, T.; Kurimoto, M.; Tsujisaka, Y.
    Purification and properties of a novel enzyme, maltooligosyl trehalose synthase, from Arthrobacter sp. Q36 (1995), Biosci. Biotechnol. Biochem., 59, 2210-2214.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ CuSO4 Arthrobacter sp.
FeSO4
-
Arthrobacter sp.
Hg2+ HgCl2 Arthrobacter sp.
MgCl2 weak Arthrobacter sp.
ZnSO4 weak Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
maltoheptaose
-
Arthrobacter sp.
1.4
-
maltohexaose
-
Arthrobacter sp.
22.9
-
maltotetraose
-
Arthrobacter sp.
8.7
-
maltopentaose
-
Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
x * 81000, SDS-PAGE Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
Q36
-
Arthrobacter sp. Q36
-
Q36
-

Purification (Commentary)

Purification (Comment) Organism
-
Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
203
-
-
Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no reaction with maltose Arthrobacter sp. ?
-
?
additional information no reaction with maltose Arthrobacter sp. Q36 ?
-
?
Maltopentaose
-
Arthrobacter sp. alpha-Maltotriosyl trehalose
-
?
Maltopentaose
-
Arthrobacter sp. Q36 alpha-Maltotriosyl trehalose
-
?
Maltoheptaose 58% of the activity with maltopentaose Arthrobacter sp. ?
-
?
Maltoheptaose 58% of the activity with maltopentaose Arthrobacter sp. Q36 ?
-
?
Maltohexaose 45% of the activity with maltopentaose Arthrobacter sp. ?
-
?
Maltohexaose 45% of the activity with maltopentaose Arthrobacter sp. Q36 ?
-
?
Maltotetraose 25% of the activity with maltopentaose Arthrobacter sp. ?
-
?
Maltotetraose 25% of the activity with maltopentaose Arthrobacter sp. Q36 ?
-
?

Subunits

Subunits Comment Organism
? x * 81000, SDS-PAGE Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Arthrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 30°C: about 80% of maximal activity, 45°C: about 85% of maximal activity Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
60 min, stable up to Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 pH 5.5: about 80% of maximal activity, pH 8.0: about 60% of maximal activity Arthrobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9.5 4°C, 24 h, stable Arthrobacter sp.