Literature summary for 5.4.99.11 extracted from

Mu, W.; Li, W.; Wang, X.; Zhang, T.; Jiang, B.
Current studies on sucrose isomerase and biological isomaltulose production using sucrose isomerase (2014), Appl. Microbiol. Biotechnol., 98, 6569-6582 .

Search for more literature for 5.4.99.11

Application

Application	Comment	Organism
additional information	in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement	Erwinia rhapontici
additional information	in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement	Serratia plymuthica
additional information	in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement	Pantoea dispersa
additional information	in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement	Raoultella planticola
additional information	in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement	Paraburkholderia acidicola
additional information	in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement	Enterobacter sp. FMB-1
additional information	in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement	Klebsiella sp. LX3
additional information	in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement	Rhizobium sp. MX-45
additional information	in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement	Klebsiella pneumoniae
additional information	in contrast to sucrose, isomaltulose is scarcely fermented by oral microbes and effectively inhibits the formation of water-insoluble glucans, showing that it is particularly suitable as a noncariogenic sucrose replacement	Erwinia sp. D12
synthesis	isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose	Erwinia rhapontici
synthesis	isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose	Serratia plymuthica
synthesis	isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose	Pantoea dispersa
synthesis	isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose	Raoultella planticola
synthesis	isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose	Paraburkholderia acidicola
synthesis	isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose	Enterobacter sp. FMB-1
synthesis	isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose	Klebsiella sp. LX3
synthesis	isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose	Rhizobium sp. MX-45
synthesis	isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose	Klebsiella pneumoniae
synthesis	isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose	Erwinia sp. D12

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant enzyme expression	Enterobacter sp. FMB-1
recombinant enzyme expression	Erwinia rhapontici
recombinant enzyme expression	Klebsiella sp. LX3
recombinant enzyme expression	Klebsiella pneumoniae
recombinant enzyme expression from tac vector	Pantoea dispersa
recombinant enzyme expression from tac vector	Raoultella planticola
recombinant enzyme expression from tac vector	Erwinia rhapontici
recombinant enzyme expression, Protaminobacter sucrose isomerase is totally susceptible to inactivation and cannot be solubly expressed with a tac vector either but may fold correctly and is effectively expressed from the L-rhamnose-inducible vector	Serratia plymuthica

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure determination, PDB ID 1M53	Klebsiella sp. LX3
crystal structure determination, PDB IDs GBD and GBE, with ethylene glycol, citrate, 1,2-ethanediol, and 1-deoxynojirimycin as interacting compounds	Serratia plymuthica
crystal structure determinations of enzyme mutants F164L and R284C, PDB IDs 4H2C, 4GIN, 4GI6, 4GI8, 4GIA, and 4GI9, interacting compounds are Ca2+, glycerin, D-glucose, and Tris	Rhizobium sp. MX-45
crystal structure determinations of native enzyme and enzyme mutants D200A and E254Q, PDB IDs 1ZJB, 2PWH, 1ZJA, 2PWD, 2PWG, 2PWF, and 2PWE, interacting compounds are Ca2+, sucrose, D-glucose, tromethamine, Tris, 1-deoxynojirimycin, and castanospermine	Paraburkholderia acidicola

Protein Variants

Protein Variants	Comment	Organism
D200A	enzyme mutant structure analysis	Paraburkholderia acidicola
E254Q	enzyme mutant structure analysis	Paraburkholderia acidicola
F164L	enzyme mutant structure analysis	Rhizobium sp. MX-45
additional information	immobilization of cells expressing the recombinant wild-type enzyme on alginate cell pellets for isomaltulose production, overview. The operational stability and enzyme half-life are significantly improved	Serratia plymuthica
additional information	immobilization of cells expressing the recombinant wild-type enzyme, and mutant BN 68069 cells, on alginate cell pellets for isomaltulose production, overview. The operational stability and enzyme half-life are significantly improved	Erwinia rhapontici
additional information	immobilization of cells expressing the wild-type enzyme on alginate cell pellets for isomaltulose production, overview. The operational stability and enzyme half-life are significantly improved	Serratia plymuthica
additional information	immobilization of cells expressing the wild-type enzyme on alginate cell pellets for isomaltulose production, overview. The operational stability and enzyme half-life are significantly improved	Erwinia rhapontici
additional information	immobilization of cells expressing the wild-type enzyme on alginate cell pellets for isomaltulose production, overview. The operational stability and enzyme half-life are significantly improved	Erwinia sp. D12
R284C	enzyme mutant structure analysis	Rhizobium sp. MX-45

Inhibitors

Inhibitors	Comment	Organism
Ag+	inactivation	Erwinia rhapontici
Ag+	inactivation	Erwinia sp. D12
Ag+	inactivation	Klebsiella pneumoniae
Hg2+	inactivation	Erwinia rhapontici
Hg2+	inactivation	Erwinia sp. D12
Hg2+	inactivation	Klebsiella pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.0192	-	sucrose	pH not specified in the publication, 20°C, native enzyme	Paraburkholderia acidicola
0.03	-	sucrose	pH and temperature not specified in the publication, recombinant enzyme	Paraburkholderia acidicola
0.0324	-	sucrose	pH and temperature not specified in the publication, recombinant enzyme	Serratia plymuthica
0.04	-	sucrose	recombinant enzyme, pH not specified in the publication, 35°C	Pantoea dispersa
0.0427	-	sucrose	recombinant enzyme, pH not specified in the publication, 35°C	Klebsiella pneumoniae
0.049	-	sucrose	pH not specified in the publication, 50°C, recombinant enzyme	Enterobacter sp. FMB-1
0.0546	-	sucrose	recombinant enzyme, pH not specified in the publication, 35°C	Klebsiella sp. LX3
0.065	-	sucrose	native enzyme, pH not specified in the publication, 30°C	Serratia plymuthica
0.076	-	sucrose	recombinant enzyme, pH not specified in the publication, 35°C	Raoultella planticola
0.138	-	sucrose	pH not specified in the publication, 40°C, native enzyme	Erwinia sp. D12
0.2	-	sucrose	recombinant enzyme, pH not specified in the publication, 30°C	Erwinia rhapontici
0.222	-	sucrose	pH not specified in the publication, 30°C, native enzyme	Erwinia rhapontici
0.257	-	sucrose	pH not specified in the publication, 30°C, recombinant enzyme	Erwinia rhapontici
0.28	-	sucrose	pH not specified in the publication, 30°C, native enzyme	Erwinia rhapontici

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
additional information	Paraburkholderia acidicola	the isomaltulose-producing enzyme from Pseudomonas mesoacidophila MX-45 shows the main product trehalulose. It is also named alpha-glucosyltransferase and is renamed trehalulose synthase	?	-	?
additional information	Rhizobium sp. MX-45	the isomaltulose-producing enzyme from Pseudomonas mesoacidophila MX-45 shows the main product trehalulose. It is also named alpha-glucosyltransferase and is renamed trehalulose synthase	?	-	?
additional information	Paraburkholderia acidicola MX-45	the isomaltulose-producing enzyme from Pseudomonas mesoacidophila MX-45 shows the main product trehalulose. It is also named alpha-glucosyltransferase and is renamed trehalulose synthase	?	-	?
sucrose	Erwinia rhapontici	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Serratia plymuthica	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Pantoea dispersa	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Raoultella planticola	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Paraburkholderia acidicola	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Enterobacter sp. FMB-1	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Klebsiella sp. LX3	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Rhizobium sp. MX-45	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Klebsiella pneumoniae	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Erwinia sp. D12	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Erwinia rhapontici DSM 4484	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Klebsiella pneumoniae NK33-98-8	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Raoultella planticola UQ14S	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Erwinia rhapontici NCPPB 1578	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Pantoea dispersa UQ68J	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Serratia plymuthica CBS 574.77	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Erwinia rhapontici wac2928	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Paraburkholderia acidicola MX-45	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Serratia plymuthica ATCC 15928	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	Erwinia rhapontici NX-5	-	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r

Organism

Organism	UniProt	Comment	Textmining
Enterobacter sp. FMB-1	B5ABD8	-	-
Erwinia rhapontici	-	-	-
Erwinia rhapontici	D9MPF2	-	-
Erwinia rhapontici	Q6XNK5	-	-
Erwinia rhapontici	Q9AI64	-	-
Erwinia rhapontici DSM 4484	Q9AI64	-	-
Erwinia rhapontici NCPPB 1578	-	-	-
Erwinia rhapontici NX-5	D9MPF2	-	-
Erwinia rhapontici wac2928	Q6XNK5	-	-
Erwinia sp. D12	-	-	-
Klebsiella pneumoniae	Q4L2Q1	-	-
Klebsiella pneumoniae NK33-98-8	Q4L2Q1	-	-
Klebsiella sp. LX3	Q8KR84	-	-
no activity in Bemisia argentifolii	-	-	-
Pantoea dispersa	Q6XNK6	-	-
Pantoea dispersa UQ68J	Q6XNK6	-	-
Paraburkholderia acidicola	Q2PS28	-	-
Paraburkholderia acidicola MX-45	Q2PS28	-	-
Raoultella planticola	Q6XKX6	i.e. Klebsiella planticola	-
Raoultella planticola UQ14S	Q6XKX6	i.e. Klebsiella planticola	-
Rhizobium sp. MX-45	M1E1F6	-	-
Serratia plymuthica	-	-	-
Serratia plymuthica ATCC 15928	-	-	-
Serratia plymuthica CBS 574.77	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
Sucrose = 6-O-alpha-D-glucopyranosyl-D-fructofuranose	Glu295 plays a role as the general acid catalyst to protonate the oxygen of the glycosidic linkage for substrate hydrolysis, the Odelta2 of Asp241 acts as the nucleophile to attack the C1 of the D-glucosyl and forms a bond with C1 to obtain beta-glucosyl-enzyme intermediate, and the isomaltulose is produced when the O-6 of the hydrolysis product D-fructose nucleophilically binds to the C1 of the D-glucosyl group of the intermediate	Serratia plymuthica	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	optimization of enzyme production from native free cells, culture conditions optimization, overview	Erwinia rhapontici	-
additional information	optimization of enzyme production from native free cells, culture conditions optimization, overview	Erwinia sp. D12	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
13.98	-	substrate sucrose, pH not specified in the publication, 20°C, native enzyme	Paraburkholderia acidicola
19.8	-	pH not specified in the publication, 40°C, native enzyme	Erwinia sp. D12
120	-	native enzyme, pH not specified in the publication, 30°C	Serratia plymuthica
328	-	recombinant enzyme, pH not specified in the publication, 35°C	Klebsiella sp. LX3
351	-	recombinant enzyme, pH not specified in the publication, 35°C	Raoultella planticola
423	-	pH not specified in the publication, 30°C, native enzyme	Erwinia rhapontici
562	-	recombinant enzyme, pH not specified in the publication, 35°C	Pantoea dispersa
900	-	substrate sucrose, pH and temperature not specified in the publication, recombinant enzyme	Paraburkholderia acidicola
2362	-	recombinant enzyme, pH not specified in the publication, 35°C	Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	the isomaltulose-producing enzyme from Pseudomonas mesoacidophila MX-45 shows the main product trehalulose. It is also named alpha-glucosyltransferase and is renamed trehalulose synthase	Paraburkholderia acidicola	?	-	?
additional information	the isomaltulose-producing enzyme from Pseudomonas mesoacidophila MX-45 shows the main product trehalulose. It is also named alpha-glucosyltransferase and is renamed trehalulose synthase	Rhizobium sp. MX-45	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Erwinia rhapontici	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Serratia plymuthica	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Pantoea dispersa	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Raoultella planticola	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Paraburkholderia acidicola	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Enterobacter sp. FMB-1	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Klebsiella sp. LX3	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Rhizobium sp. MX-45	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Klebsiella pneumoniae	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Erwinia sp. D12	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Erwinia rhapontici DSM 4484	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Klebsiella pneumoniae NK33-98-8	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Raoultella planticola UQ14S	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Erwinia rhapontici NCPPB 1578	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Pantoea dispersa UQ68J	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Serratia plymuthica CBS 574.77	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Erwinia rhapontici wac2928	?	-	?
additional information	the isomaltulose-producing enzyme from Pseudomonas mesoacidophila MX-45 shows the main product trehalulose. It is also named alpha-glucosyltransferase and is renamed trehalulose synthase	Paraburkholderia acidicola MX-45	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Paraburkholderia acidicola MX-45	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Serratia plymuthica ATCC 15928	?	-	?
additional information	the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides	Erwinia rhapontici NX-5	?	-	?
sucrose	-	Erwinia rhapontici	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Serratia plymuthica	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Pantoea dispersa	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Raoultella planticola	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Paraburkholderia acidicola	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Enterobacter sp. FMB-1	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Klebsiella sp. LX3	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Rhizobium sp. MX-45	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Klebsiella pneumoniae	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Erwinia sp. D12	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Erwinia rhapontici DSM 4484	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Klebsiella pneumoniae NK33-98-8	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Raoultella planticola UQ14S	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Erwinia rhapontici NCPPB 1578	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Pantoea dispersa UQ68J	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Serratia plymuthica CBS 574.77	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Erwinia rhapontici wac2928	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Paraburkholderia acidicola MX-45	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Serratia plymuthica ATCC 15928	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r
sucrose	-	Erwinia rhapontici NX-5	6-O-alpha-D-Glucopyranosyl-D-fructofuranose	-	r

Subunits

Subunits	Comment	Organism
?	x * 63000, native enzyme	Erwinia sp. D12
?	x * 64300, recombinant enzyme, x * 63000, native enzyme	Paraburkholderia acidicola
?	x * 65000, native enzyme	Serratia plymuthica
?	x * 65600, native enzyme, x * 66000, recombinant enzyme	Erwinia rhapontici
?	x * 69940, recombinant enzyme	Klebsiella sp. LX3
?	x * 69980, recombinant enzyme	Klebsiella pneumoniae
?	x * 70000, recombinant enzyme	Enterobacter sp. FMB-1

Synonyms

Synonyms	Comment	Organism
Sucrose alpha-glucosyltransferase	-	Erwinia rhapontici
Sucrose alpha-glucosyltransferase	-	Serratia plymuthica
Sucrose alpha-glucosyltransferase	-	Pantoea dispersa
Sucrose alpha-glucosyltransferase	-	Raoultella planticola
Sucrose alpha-glucosyltransferase	-	Paraburkholderia acidicola
Sucrose alpha-glucosyltransferase	-	Enterobacter sp. FMB-1
Sucrose alpha-glucosyltransferase	-	Klebsiella sp. LX3
Sucrose alpha-glucosyltransferase	-	Rhizobium sp. MX-45
Sucrose alpha-glucosyltransferase	-	Klebsiella pneumoniae
Sucrose alpha-glucosyltransferase	-	Erwinia sp. D12
sucrose isomerase	-	Erwinia rhapontici
sucrose isomerase	-	Serratia plymuthica
sucrose isomerase	-	Pantoea dispersa
sucrose isomerase	-	Raoultella planticola
sucrose isomerase	-	Paraburkholderia acidicola
sucrose isomerase	-	Enterobacter sp. FMB-1
sucrose isomerase	-	Klebsiella sp. LX3
sucrose isomerase	-	Rhizobium sp. MX-45
sucrose isomerase	-	Klebsiella pneumoniae
sucrose isomerase	-	Erwinia sp. D12
Trehalulose synthase	-	Erwinia rhapontici
Trehalulose synthase	-	Serratia plymuthica
Trehalulose synthase	-	Pantoea dispersa
Trehalulose synthase	-	Raoultella planticola
Trehalulose synthase	-	Paraburkholderia acidicola
Trehalulose synthase	-	Enterobacter sp. FMB-1
Trehalulose synthase	-	Klebsiella sp. LX3
Trehalulose synthase	-	Rhizobium sp. MX-45
Trehalulose synthase	-	Klebsiella pneumoniae
Trehalulose synthase	-	Erwinia sp. D12

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	native enzyme	Paraburkholderia acidicola
30	-	-	Erwinia rhapontici
30	-	-	Serratia plymuthica
30	-	native and recombinant enzyme	Erwinia rhapontici
35	-	-	Pantoea dispersa
35	-	-	Raoultella planticola
35	-	-	Klebsiella sp. LX3
40	-	-	Erwinia sp. D12
50	-	recombinant enzyme	Enterobacter sp. FMB-1

pI Value

Organism	Comment	pI Value Maximum	pI Value
Paraburkholderia acidicola	native enzyme	-	5.4
Klebsiella pneumoniae	recombinant enzyme	-	5.89
Erwinia rhapontici	native enzyme	-	6
Enterobacter sp. FMB-1	recombinant enzyme	-	6.02
Klebsiella sp. LX3	recombinant enzyme	-	6.62
Serratia plymuthica	-	-	9

General Information

General Information	Comment	Organism
additional information	the unique RLDRD motif is located in a loop adjacent to the active site cleft and is determined to play an important role in the isomerization process and in the control of product specificity	Serratia plymuthica
physiological function	physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview	Erwinia rhapontici
physiological function	physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview	Serratia plymuthica
physiological function	physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview	Pantoea dispersa
physiological function	physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview	Raoultella planticola
physiological function	physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview	Paraburkholderia acidicola
physiological function	physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview	Enterobacter sp. FMB-1
physiological function	physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview	Klebsiella sp. LX3
physiological function	physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview	Rhizobium sp. MX-45
physiological function	physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview	Klebsiella pneumoniae
physiological function	physiological functions of isomaltulose and biochemical properties of sucrose isomerases, as well as biological isomaltulose production from sucrose using sucrose isomerase, overview	Erwinia sp. D12

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
6.19	-	sucrose	recombinant enzyme, pH not specified in the publication, 35°C	Raoultella planticola
17.9	-	sucrose	recombinant enzyme, pH not specified in the publication, 35°C	Pantoea dispersa
36	-	sucrose	pH and temperature not specified in the publication, recombinant enzyme	Paraburkholderia acidicola
1301	-	sucrose	pH and temperature not specified in the publication, recombinant enzyme	Serratia plymuthica