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Literature summary for 5.4.99.11 extracted from
- Functional characterization of the sucrose isomerase responsible for trehalulose production in plant-associated Pectobacterium species (2014), Enzyme Microb. Technol., 55, 100-106.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons, functional expression of His6-tagged enzyme in Escherichia coli | Pectobacterium carotovorum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68800 | - |
x * 68800, about, sequence calculation | Pectobacterium carotovorum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose | Pectobacterium carotovorum | production of trehalulose and isomaltulose in a ratio of 64:36 | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? |  |
| sucrose | Pectobacterium carotovorum KKH 3-1 | production of trehalulose and isomaltulose in a ratio of 64:36 | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pectobacterium carotovorum | S5YEW8 | - |
- |
| Pectobacterium carotovorum KKH 3-1 | S5YEW8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli by nickle affinity chromatography and gel filtration | Pectobacterium carotovorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose | production of trehalulose and isomaltulose in a ratio of 64:36 | Pectobacterium carotovorum | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? | |
| sucrose | production of trehalulose and isomaltulose in a ratio of 64:36 at 40°C. The product ratio depends on the reaction temperature, overview. The amount of trehalulose increases from 47.5% to 79.1% as temperature is lowered from 50°C to 30°C | Pectobacterium carotovorum | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? | |
| sucrose | production of trehalulose and isomaltulose in a ratio of 64:36 | Pectobacterium carotovorum KKH 3-1 | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? | |
| sucrose | production of trehalulose and isomaltulose in a ratio of 64:36 at 40°C. The product ratio depends on the reaction temperature, overview. The amount of trehalulose increases from 47.5% to 79.1% as temperature is lowered from 50°C to 30°C | Pectobacterium carotovorum KKH 3-1 | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 68800, about, sequence calculation | Pectobacterium carotovorum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SIase | - |
Pectobacterium carotovorum |
| sucrose isomerase | - |
Pectobacterium carotovorum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pectobacterium carotovorum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pectobacterium carotovorum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pectobacterium carotovorum | sequence calculation | - |
5.96 |
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Release 2023.1 (January 2023)
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