Application | Comment | Organism |
---|---|---|
food industry | sucrose isomerase activity is used industrially for the conversion of sucrose into isomers, particularly isomaltulose or trehalulose, which have properties advantageous over sucrose for some food uses. The industrial potential may be further enhanced by selection for variants that do not catabolize the sucrose substrate | Rhizobium sp. |
food industry | sucrose isomerase activity is used industrially for the conversion of sucrose into isomers, particularly isomaltulose or trehalulose, which have properties advantageous over sucrose for some food uses.The industrial potential may be further enhanced by selection for variants that do not catabolize the sucrose substrate | Serratia plymuthica |
Cloned (Comment) | Organism |
---|---|
gene mutB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, cloning and expression of wild-type and insertional disruption mutant in Escherichia coli strain BL21 (DE3) | Rhizobium sp. |
gene smuA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, cloning of full-length gene including the periplasmic signal sequence and expression of wild-type and insertional disruption mutant in Escherichia coli strain BL21 (DE3) | Serratia plymuthica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose | Serratia plymuthica | - |
1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? | |
sucrose | Rhizobium sp. | - |
1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | high product specificity for trehalulose (alpha-D-glucosylpyranosyl-1,6-D-fructofuranose) | ? | |
sucrose | Serratia plymuthica L0W308 | - |
1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium sp. | M1E1F3 | i.e. Pseudomonas mesoacidophila MX-45, gene mutB | - |
Rhizobium sp. | M1E1F6 | i.e. Pseudomonas mesoacidophila MX-45, gene mutB | - |
Rhizobium sp. | M1E1F7 | i.e. Pseudomonas mesoacidophila MX-45, gene mutB | - |
Serratia plymuthica | D0VX20 | i.e. Protaminobacter rubrum CBS 574.77, gene smuA | - |
Serratia plymuthica L0W308 | D0VX20 | i.e. Protaminobacter rubrum CBS 574.77, gene smuA | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) | Serratia plymuthica |
recombinant enzyme from Escherichia coli strain BL21(DE3) | Rhizobium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose | - |
Serratia plymuthica | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? | |
sucrose | - |
Rhizobium sp. | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? | |
sucrose | - |
Rhizobium sp. | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | high product specificity for trehalulose (alpha-D-glucosylpyranosyl-1,6-D-fructofuranose) | ? | |
sucrose | - |
Serratia plymuthica L0W308 | 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sucrose isomerase | - |
Serratia plymuthica |
sucrose isomerase | - |
Rhizobium sp. |