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Literature summary for 5.4.99.11 extracted from
- Cloning and characterization of a sucrose isomerase from Erwinia rhapontici NX-5 for isomaltulose hyperproduction (2011), Appl. Biochem. Biotechnol., 163, 52-63.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Erwinia rhapontici |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 20-30% inhibition at 1 mM | Erwinia rhapontici |  |
| Co2+ | 20-30% inhibition at 1 mM | Erwinia rhapontici | |
| Cu2+ | 20-30% inhibition at 1 mM | Erwinia rhapontici | |
| EDTA | 18% inhibition at 5 mM | Erwinia rhapontici | |
| Zn2+ | 20-30% inhibition at 1 mM | Erwinia rhapontici | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 257 | - |
sucrose | in 50 mM PBS (pH 6.0), at 30°C | Erwinia rhapontici | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates | Erwinia rhapontici | |
| additional information | Mg2+ and Zn2+ show no significant influence on enzyme activity | Erwinia rhapontici |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 66000, SDS-PAGE | Erwinia rhapontici |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Erwinia rhapontici | D9MPF2 | - |
- |
| Erwinia rhapontici NX-5 | D9MPF2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Erwinia rhapontici |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose | 99% conversion | Erwinia rhapontici | isomaltulose | - |
? | |
| sucrose | 99% conversion | Erwinia rhapontici NX-5 | isomaltulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, SDS-PAGE | Erwinia rhapontici |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SIase | - |
Erwinia rhapontici |
| sucrose isomerase | - |
Erwinia rhapontici |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Erwinia rhapontici |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | the enzyme is stable for longer than 48 h at 30°C. The stability of the enzyme decreases drastically at 50°C and 60°C with a half-life duration of 90 min and 5 min, respectively | Erwinia rhapontici |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Erwinia rhapontici |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | 89%, 86%, 80%, 34%, and 30% of maximum activity at pH 4.0, pH 6.0, pH 7.0, pH 8.0, and pH 9.0 | Erwinia rhapontici |
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