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Literature summary for 5.4.99.11 extracted from
- Isomaltulose production from sucrose by Protaminobacter rubrum immobilized in calcium alginate (2009), Biores. Technol., 100, 4252-4256.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sucrose | the production of isomaltulose with alginate-immobilized Protaminobacter rubrum at 30 °C is influenced by sucrose concentration. Isomaltulose yields above 90% in 8 h are reached in 40-65% sucrose solutions, and yields up to 82% in 24 h were obtained with 70% sucrose. At higher concentrations (75%) a significant decrease in the isomaltulose yield (42.7-55%) is observed | Serratia plymuthica |  |
General Stability
| General Stability | Organism |
|---|---|
| the isomaltulose synthase immobilized only with alginate remains stable for eight 24 h batch cycles or 192 h total time | Serratia plymuthica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Glutaraldehyde | the application of 1 or 2% glutaraldehyde after immobilization of Protaminobacter rubrum in alginate results in the complete destruction of the isomaltulose synthase activity | Serratia plymuthica | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Serratia plymuthica | - |
- |
- |
| Serratia plymuthica CBS 574.77 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the specific activity of the Protaminobacter rubrum immobilized pellets in calcium alginate at 30°C range from 1.6 to 4.0 g isomaltulose/g pellet/h, respectively with 70% and 65% sucrose solution, while in lower sucrose concentration have higher specific activities presumably due to substrate inhibition of the isomaltulose synthase in higher sucrose concentrations | Serratia plymuthica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose | - |
Serratia plymuthica | isomaltulose | 89-94% yield | ? | |
| sucrose | - |
Serratia plymuthica CBS 574.77 | isomaltulose | 89-94% yield | ? | |
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Release 2023.1 (January 2023)
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