Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
D140E | almost complete loss of catalytic activity | Klebsiella pneumoniae |
D140G | almost complete loss of catalytic activity | Klebsiella pneumoniae |
D140N | almost complete loss of catalytic activity | Klebsiella pneumoniae |
D329A | 42% of wild-type activity, no change in major products | Klebsiella pneumoniae |
L326Y | 20% of wild-type activity, no change in major products | Klebsiella pneumoniae |
L326Y/D329A | 3.4% of wild-type activity, no change in major products | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 1 mM, complete inhibition | Klebsiella pneumoniae | |
sodium dodecylsulfate | 1 mM, complete inhibition | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
42.7 | - |
sucrose | pH 6.0, 30°C | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
x * 69980, calculated, x * 67000, SDS-PAGE | Klebsiella pneumoniae |
69980 | - |
x * 69980, calculated, x * 67000, SDS-PAGE | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae NK33-98-8 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2362 | - |
pH 6.0, 30°C | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme does not catalyze the formation of any disaccharide from glucose and fructose. No trehalulose is detected when palatinose is the substrate and vice versa | Klebsiella pneumoniae | ? | - |
? | |
additional information | enzyme does not catalyze the formation of any disaccharide from glucose and fructose. No trehalulose is detected when palatinose is the substrate and vice versa | Klebsiella pneumoniae NK33-98-8 | ? | - |
? | |
sucrose | - |
Klebsiella pneumoniae | palatinose + trehalulose + D-glucose + D-fructose | at 30°C, 76.8% palatinose, 21.2% trehalulose, 1% each of D-glucose and D-fructose. Percentage of palatinose increases with temperature and reaches a maximum of 79.7% at 40°C | ir | |
sucrose | - |
Klebsiella pneumoniae NK33-98-8 | palatinose + trehalulose + D-glucose + D-fructose | at 30°C, 76.8% palatinose, 21.2% trehalulose, 1% each of D-glucose and D-fructose. Percentage of palatinose increases with temperature and reaches a maximum of 79.7% at 40°C | ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 69980, calculated, x * 67000, SDS-PAGE | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Klebsiella pneumoniae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
25% residual activity | Klebsiella pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable for at least 1 h | Klebsiella pneumoniae |
50 | - |
20 min, loss of 40% of activity | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Klebsiella pneumoniae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | - |
80% residual activity | Klebsiella pneumoniae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 1 h, more than 80% residual activity | Klebsiella pneumoniae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Klebsiella pneumoniae | calculated | - |
5.9 |