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Literature summary for 5.4.99.11 extracted from

  • Ravaud, S.; Watzlawick, H.; Haser, R.; Mattes, R.; Aghajari, N.
    Expression, purification, crystallization and preliminary x-ray crystallographic studies of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45 (2005), Acta Crystallogr. Sect. F, F61, 100-103.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paraburkholderia acidicola

Crystallization (Commentary)

Crystallization (Comment) Organism
expression in Escherichia coli Paraburkholderia acidicola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 x * 64000, SDS-PAGE and calculated Paraburkholderia acidicola

Organism

Organism UniProt Comment Textmining
Paraburkholderia acidicola
-
 stain MX-45
-
Paraburkholderia acidicola MX-45
-
 stain MX-45
-

Purification (Commentary)

Purification (Comment) Organism
expression in Escherichia coli Paraburkholderia acidicola

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
900
-
 pH 5.5, 25°C Paraburkholderia acidicola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose
-
 Paraburkholderia acidicola 6-O-alpha-D-Glucopyranosyl-D-fructofuranose
-
 ?
sucrose
-
 Paraburkholderia acidicola MX-45 6-O-alpha-D-Glucopyranosyl-D-fructofuranose
-
 ?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE and calculated Paraburkholderia acidicola