Cloned (Comment) | Organism |
---|---|
- |
Klebsiella sp. |
Protein Variants | Comment | Organism |
---|---|---|
E498P | temperature-optimum is 40°C compared to 35°C for the wild-type enzyme, maximal specific activity increases by 7%. Half-life at 50°C is 9.45 min compared to 1.81 min for wild-type enzyme. Mutation slightly increases the ratio of turnover number to KM-value. Percent content of monosaccharide decreases from 5.9% of the wild-type enzyme to 3.4% | Klebsiella sp. |
E498P/R310P | temperature-optimum is 45°C compared to 35°C for the wild-type enzyme, maximal specific activity increases by 16%. Half-life is 13.61 min compared to 1.81 min for wild-type enzyme. Mutation slightly increases the ratio of turnover number to KM-value. Percent content of monosaccharide decreases from 5.9% of the wild-type enzyme to 3.3% | Klebsiella sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO3 | 14% inhibition at 1 mM, 23% inhibition at 5 mM | Klebsiella sp. | |
EDTA | 36% inhibition at 3 mM, 70% inhibition at 15 mM | Klebsiella sp. | |
Fe3+ | 5 mM FeCl3, complete inactivation | Klebsiella sp. | |
FeCl2 | 25% inhibition at 1 mM, 51% inhibition at 5 mM | Klebsiella sp. | |
Hg2+ | 1 mM HgCl2, complete inactivation | Klebsiella sp. | |
SDS | 3 mM, complete inactivation | Klebsiella sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
54.6 | - |
sucrose | pH 6.0, 35°C | Klebsiella sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | enhances activity | Klebsiella sp. | |
Mn2+ | enhances activity | Klebsiella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose | - |
Klebsiella sp. | alpha-D-glucopyranosyl-1,6-D-fructofuranose + alpha-D-glucopyranosyl-1,1-D-fructofuranose | the major product is alpha-D-glucopyranosyl-1,6-D-fructofuranose, small amounts of alpha-D-glucopyranosyl-1,1-D-fructofuranose | ? |
Synonyms | Comment | Organism |
---|---|---|
PalI | - |
Klebsiella sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
wild-type enzyme | Klebsiella sp. |
40 | - |
mutant enzyme E498P | Klebsiella sp. |
45 | - |
mutant enzyme E498P/R310P | Klebsiella sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life of wild-type enzyme: 1.81 min, half-life of mutant enzymes E498P is 9.45 min, half-life of mutant enzyme E498P/R310P is 13.61 min | Klebsiella sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Klebsiella sp. |