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Literature summary for 5.4.99.1 extracted from

  • Barker, H.A.
    Glutamate mutase (1969), Methods Enzymol., 13, 319-330.
No PubMed abstract available
Search for more literature for 5.4.99.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 L-threo-3-methylaspartate
-
 Clostridium tetanomorphum
1.5
-
 L-Glu
-
 Clostridium tetanomorphum

Organism

Organism UniProt Comment Textmining
Clostridium tetanomorphum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of component E and component S Clostridium tetanomorphum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Glu
-
 Clostridium tetanomorphum threo-3-Methylaspartate
-
 ?
L-threo-3-methylaspartate
-
 Clostridium tetanomorphum L-glutamate
-
 ?

Subunits

Subunits Comment Organism
? the enzyme consists of two separable proteins called component E and component S, MW of component E determined by ultracentrifugation is 128000, the molecular weight of component S determined by ultracentrifugation is 17000 Clostridium tetanomorphum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Clostridium tetanomorphum
40
-
 maximal activity in a 30 min incubation period Clostridium tetanomorphum
55
-
 maximal activity in an incubation period that is shorter than 30 min Clostridium tetanomorphum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 37 the reaction rate increases about 3fold when the temperature is increased from 25°C to 37°C Clostridium tetanomorphum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Clostridium tetanomorphum

Cofactor

Cofactor Comment Organism Structure
Co-Deoxycobalamin essential for the reaction Clostridium tetanomorphum