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Literature summary for 5.4.4.8 extracted from

  • Marmulla, R.; Safaric, B.; Markert, S.; Schweder, T.; Harder, J.
    Linalool isomerase, a membrane-anchored enzyme in the anaerobic monoterpene degradation in Thauera linaloolentis 47Lol (2016), BMC Biochem., 17, 006 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.4.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thauera linaloolentis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.455
-
 geraniol pH 8.0, 35°C Thauera linaloolentis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane inner membrane. Protein is predicted to have four transmembrane helices at the N-terminal domain and a cytosolic domain Thauera linaloolentis 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-linalool Thauera linaloolentis
-
 geraniol
-
 r
(R)-linalool Thauera linaloolentis DSM 12138
-
 geraniol
-
 r
(S)-linalool Thauera linaloolentis
-
 geraniol
-
 r
(S)-linalool Thauera linaloolentis DSM 12138
-
 geraniol
-
 r
geraniol Thauera linaloolentis
-
 (R)-linalool + (S)-linalool
-
 r
geraniol Thauera linaloolentis DSM 12138
-
 (R)-linalool + (S)-linalool
-
 r

Organism

Organism UniProt Comment Textmining
Thauera linaloolentis N6Z5E2
-
-
Thauera linaloolentis DSM 12138 N6Z5E2
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
57
-
 pH 8.0, 35°C Thauera linaloolentis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-linalool
-
 Thauera linaloolentis geraniol
-
 r
(R)-linalool
-
 Thauera linaloolentis DSM 12138 geraniol
-
 r
(S)-linalool
-
 Thauera linaloolentis geraniol
-
 r
(S)-linalool
-
 Thauera linaloolentis DSM 12138 geraniol
-
 r
geraniol
-
 Thauera linaloolentis (R)-linalool + (S)-linalool
-
 r
geraniol
-
 Thauera linaloolentis DSM 12138 (R)-linalool + (S)-linalool
-
 r
additional information presence of a reductant is required, maximum activity in presence of 4 mM dithionite Thauera linaloolentis ?
-
 ?
additional information presence of a reductant is required, maximum activity in presence of 4 mM dithionite Thauera linaloolentis DSM 12138 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 71800, calculated, x * 60000-70000, SDS-PAGE Thauera linaloolentis

Synonyms

Synonyms Comment Organism
C666_11245
-
 Thauera linaloolentis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Thauera linaloolentis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Thauera linaloolentis

pI Value

Organism Comment pI Value Maximum pI Value
Thauera linaloolentis calculated
-
 6.1

General Information

General Information Comment Organism
physiological function strain is able to use the tertiary monoterpene alcohol (R,S)-linalool as sole carbon and energy source under denitrifying conditions Thauera linaloolentis