Literature summary for 5.4.4.2 extracted from

Parsons, J.F.; Shi, K.M.; Ladner, J.E.
Structure of isochorismate synthase in complex with magnesium (2008), Acta Crystallogr. Sect. D, 64, 607-610.

Search for more literature for 5.4.4.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BL21 (DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme without Mg2+, hanging drop vapour diffusion method, using 0.2 M trisodium citrate, 6% glycerol, 8% PEG 3350, enzyme in complex with Mg2+, sitting drop vapour diffusion method, using 0.1 M bis-tris pH 6.5, 20% PEG MME 5000	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for catalysis	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P38051	-	-

Purification (Commentary)

Purification (Comment)	Organism
Co2+-ion affinity chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Chorismate	-	Escherichia coli	Isochorismate	-	ir

Synonyms

Synonyms	Comment	Organism
menaquinone-specific isochorismate synthase	-	Escherichia coli
MenF	-	Escherichia coli

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Release 2023.1 (January 2023)