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Literature summary for 5.4.4.2 extracted from

  • Leduc, C.; Birgel, R.; Muller, R.; Leistner, E.
    Isochorismate hydroxymutase from a cell-suspension culture of Galium mollugo L. (1997), Planta, 202, 206-210.
No PubMed abstract available

General Stability

General Stability Organism
no activity in presence of 20 mM ascorbate or 5 mM thiourea. 43% decrease in activity in presence of 10 mM Cys Galium mollugo

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.675
-
isochorismate
-
Galium mollugo
0.807
-
chorismate
-
Galium mollugo

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement Galium mollugo

Organism

Organism UniProt Comment Textmining
Galium mollugo
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Galium mollugo

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Galium mollugo
-

Storage Stability

Storage Stability Organism
4°C or -20°C, 50% loss of activity after 4 days Galium mollugo

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate
-
Galium mollugo Isochorismate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Galium mollugo

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.8 about 50% of maximal activity at pH 5.5 and at pH 8.8 Galium mollugo