Literature summary for 5.4.4.1 extracted from

Davis, J.K.; Paoli, G.C.; He, Z.; Nadeau, L.J.; Somerville, C.C.; Spain, J.C.
Sequence analysis and initial characterization of two isozymes of hydroxylaminobenzene mutase from Pseudomonas pseudoalcaligenes JS45 (2000), Appl. Environ. Microbiol., 66, 2965-2971.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of habA and habB in Escherichia coli	Pseudomonas oleovorans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	not an integral membrane protein	Pseudomonas oleovorans	-	-

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas oleovorans	-	-	-
Pseudomonas oleovorans	-	encoded on 2 genes, habA and habB, only habA is expressed	-
Pseudomonas oleovorans JS45	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant HabB and HabA	Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(hydroxyamino)benzene	highly selective for the production of the ortho-isomer	Pseudomonas oleovorans	2-aminophenol	highly selective for the production of the ortho-isomer	?
(hydroxyamino)benzene	highly selective for the production of the ortho-isomer	Pseudomonas oleovorans JS45	2-aminophenol	highly selective for the production of the ortho-isomer	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	recombinant HabA, inactivation above, 50% loss of activity at 70°C after 10 min	Pseudomonas oleovorans
85	-	recombinant HabB, no loss of activity after 10 min	Pseudomonas oleovorans

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Release 2023.1 (January 2023)