Cloned (Comment) | Organism |
---|---|
gene pa4088, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged wild-type enzyme and enzyme mutant K286A in complex with pyridoxamine 5'-phosphate cofactor, mixing of 10 mg/ml protein in 20 mM Tris, 200 mM NaCl, pH 8.0, with an equal volume of reservoir solution consisting of 0.2M ammonium sulfate, 0.1 M Bis-Tris, pH 6.5, and 25% PEG 3350 for the wild-type enzyme, and of 0.1 M Tris, pH 8.5, and 25% PEG3350 for the mutant enzyme, 20°C, 1 week, X-ray diffraction structure determination and analysis at 1.44-1.57 A resolution | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
K286A | site-directed mutagenesis | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate 1-semialdehyde | Pseudomonas aeruginosa | - |
5-aminolevulinate | - |
? | |
L-glutamate 1-semialdehyde | Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 | - |
5-aminolevulinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HWU0 | - |
- |
Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 | Q9HWU0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate 1-semialdehyde | - |
Pseudomonas aeruginosa | 5-aminolevulinate | - |
? | |
L-glutamate 1-semialdehyde | - |
Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 | 5-aminolevulinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | three-dimensional structure analysis of wild-type and mutant enzymes, overview | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
glutamate-1-semialdehyde-aminomutase | - |
Pseudomonas aeruginosa |
PA4088 | - |
Pseudomonas aeruginosa |
protein PA4088 | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | in both the pyridoxamine 5'-phosphate- and pyridoxal 5'-phosphate-bound structures, the gating loops are well-defined in electron density and are in the ordered open conformation. The conformation of the gating loop is symmetrical | Pseudomonas aeruginosa | |
pyridoxal 5'-phosphate | a pyridoxamine 5'-phosphate (PMP)/pyridoxal 5'-phosphate (PLP)-dependent enzyme, binding structure with a Schiff base linkage between the cofactor and the epsilon-amino group of Lys286, overview | Pseudomonas aeruginosa | |
pyridoxamine 5'-phosphate | a pyridoxamine 5'-phosphate (PMP)/pyridoxal 5'-phosphate (PLP)-dependent enzyme, binding structure, overview | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the GSAM family | Pseudomonas aeruginosa |
additional information | three-dimensional structure analysis of wild-type and mutant enzymes, overview. In both the pyridoxamine 5'-phosphate- and pyridoxal 5'-phosphate-bound structures, the gating loops are well-defined in electron density and are in the ordered open conformation. The conformation of the gating loop is symmetrical | Pseudomonas aeruginosa |