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Literature summary for 5.4.3.8 extracted from

  • Li, S.; Lou, X.; Xu, Y.; Teng, X.; Che, S.; Liu, R.; Bartlam, M.
    Crystal structure of a glutamate-1-semialdehyde-aminomutase from Pseudomonas aeruginosa PAO1 (2018), Biochem. Biophys. Res. Commun., 500, 804-809 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene pa4088, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged wild-type enzyme and enzyme mutant K286A in complex with pyridoxamine 5'-phosphate cofactor, mixing of 10 mg/ml protein in 20 mM Tris, 200 mM NaCl, pH 8.0, with an equal volume of reservoir solution consisting of 0.2M ammonium sulfate, 0.1 M Bis-Tris, pH 6.5, and 25% PEG 3350 for the wild-type enzyme, and of 0.1 M Tris, pH 8.5, and 25% PEG3350 for the mutant enzyme, 20°C, 1 week, X-ray diffraction structure determination and analysis at 1.44-1.57 A resolution Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
K286A site-directed mutagenesis Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate 1-semialdehyde Pseudomonas aeruginosa
-
5-aminolevulinate
-
?
L-glutamate 1-semialdehyde Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
-
5-aminolevulinate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HWU0
-
-
Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Q9HWU0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate 1-semialdehyde
-
Pseudomonas aeruginosa 5-aminolevulinate
-
?
L-glutamate 1-semialdehyde
-
Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 5-aminolevulinate
-
?

Subunits

Subunits Comment Organism
dimer three-dimensional structure analysis of wild-type and mutant enzymes, overview Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
glutamate-1-semialdehyde-aminomutase
-
Pseudomonas aeruginosa
PA4088
-
Pseudomonas aeruginosa
protein PA4088
-
Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
additional information in both the pyridoxamine 5'-phosphate- and pyridoxal 5'-phosphate-bound structures, the gating loops are well-defined in electron density and are in the ordered open conformation. The conformation of the gating loop is symmetrical Pseudomonas aeruginosa
pyridoxal 5'-phosphate a pyridoxamine 5'-phosphate (PMP)/pyridoxal 5'-phosphate (PLP)-dependent enzyme, binding structure with a Schiff base linkage between the cofactor and the epsilon-amino group of Lys286, overview Pseudomonas aeruginosa
pyridoxamine 5'-phosphate a pyridoxamine 5'-phosphate (PMP)/pyridoxal 5'-phosphate (PLP)-dependent enzyme, binding structure, overview Pseudomonas aeruginosa

General Information

General Information Comment Organism
evolution the enzyme is a member of the GSAM family Pseudomonas aeruginosa
additional information three-dimensional structure analysis of wild-type and mutant enzymes, overview. In both the pyridoxamine 5'-phosphate- and pyridoxal 5'-phosphate-bound structures, the gating loops are well-defined in electron density and are in the ordered open conformation. The conformation of the gating loop is symmetrical Pseudomonas aeruginosa